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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 3052-3070
DOI: https://doi.org/10.20546/ijcmas.2018.702.369


Molecular Characterization and Evolutionary Analysis of Potential Fusarium Resistant Genes for Crop Improvement
Ashok Yadav1*, Usha Kalidindi1, Pawan Kumar Jayaswal3,Shikha Mittal2 and Nepolean Thirunavukkarasu2
1Divison of Fruits and Horticultural Technology, Indian Agricultural Research Institute,New Delhi-110012, India
2Division of Genetics, Indian Agricultural Research Institute, New Delhi-110012, India
3National Research Centre for Plant Biotechnology, Indian Agricultural Research Institute, New Delhi-110012, India
*Corresponding author
Abstract:

Fusarium species cause considerable crop losses worldwide and necessitate an expensive use of chemical pesticides. Resistant genes can be used to improve plant resistance through gene editing and gene silencing strategies. Currently, information on host defences against Fusarium species is limited. Through in-silico tools, identification of genes and understanding of disease resistance mechanism has become possible. In the present study, a comprehensive in-silico analysis of 65 Fusarium resistance genes belonging to 10 different species was carried out to understand the physiochemical properties, secondary and tertiary protein structures, protein binding sites, phylogenetic relationship, cis-regulatory elements and protein motifs. Five genes on the basis of their stability have been selected for 3D structure prediction using Phyre2 server. These structures were evaluated and validated using Verify-3D, PROCHECK and Ramachandran plot. The Z score predicted by ProSA-web tool indicated reliability of the 3D structures while above 97% of the region falls in allowed region of Ramachandran plot. The distribution pattern in five genes for binding site is high, while hydrophobicity is very low to low. The most commonly occurring motifs among Fusarium resistance genes were motif-3 and motif-1, whereas cis-regulatory elements were TC-rich repeats, Box-W1, EIRE, AT-rich sequence, CGTCA-motif and TGACG-motif. The results of cis-acting regulatory elements and motif analysis revealed that they are actively involved in the expression and regulation of Fusarium resistance genes during cellular development and under biotic stress conditions in different plant species. In-silico analysis revealed the homology of Fusarium resistance genes and will be helpful to develop molecular markers or gene specific markers to overcome the Fusarium related biotic stresses.


Keywords: Fusarium, Candidate genes, cis-regulatory, Disease resistance protein structure
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How to cite this article:

Ashok Yadav, Usha Kalidindi, Pawan Kumar Jayaswal, Shikha Mittal and Nepolean Thirunavukkarasu. 2018. Molecular Characterization and Evolutionary Analysis of Potential Fusarium Resistant Genes for Crop Improvement.Int.J.Curr.Microbiol.App.Sci. 7(2): 3052-3070. doi: https://doi.org/10.20546/ijcmas.2018.702.369