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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 2933-2941
DOI: https://doi.org/10.20546/ijcmas.2018.702.356


Staphylococcus aureus – A Versatile Pathogen Biochemical Characterization and Antibiogram
A.W. Samira1, Samith Ahmed2* and Meera D. Meundi1
1Department of Microbiology, All Saints University School of Medicine, Hillsborough Street, Roseau, Dominica, West Indies
2Department of Pathology, All Saints University School of Medicine, Hillsborough Street, Roseau, Dominica, West Indies
*Corresponding author
Abstract:

S. aureus is a major human pathogen capable of causing a wide range of infections and remains the second cause of nosocomial infections. To biochemically characterise the isolated coagulase-positive staphylococci, to determine its antimicrobial susceptibility and the MRSA status. 2220 samples were processed in the study period among which 150 were S. aureus, identified by Gram staining and coagulase test. Tests for characterisation included mannitol fermentation, demonstration of pigment production on milk agar, phosphatase test, DNase test, tellurite reduction test and urease production. Anti-microbial susceptibility was done using the commonly used antibiotics and MRSA (Methicillin- resistant Staphylococcus aureus) status was determined by cefoxitin disc diffusion method. Of the 150 tube coagulase positive S. aureus, only 145 (96.7%) were haemolytic, which were slide coagulase positive too in the first minute. The remaining 5 were slide coagulase positive after 1 minute. The positivity of 150 S. aureus to the other pathogenicity tests were mannitol fermentation (100%), DNase test (100%), pigment production on milk agar (100%), tellurite reduction (95.3%), phosphatase production (92%) and urease test (90.7%). Out of the 150 S. aureus, 54 % were MRSA. All the S. aureus isolates were resistant to ampicillin and cephalexin, partially resistant to other antibiotics and completely sensitive to vancomycin and linezolid. The MRSA isolates were resistant to cotrimoxazole, chloramphenicol, ciprofloxacin, tetracycline, gentamicin, erythromycin and clindamycin. The methicillin-sensitive strains were resistant to the above mentioned antibiotics but with a lower percentage; difference being about half the total percentage.


Keywords: S. aureus, Coagulase test, MRSA
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How to cite this article:

Samira A. W., Samith Ahmed and Meera D. Meundi. 2018. Staphylococcus aureus – A Versatile Pathogen Biochemical Characterization and Antibiogram.Int.J.Curr.Microbiol.App.Sci. 7(2): 2933-2941. doi: https://doi.org/10.20546/ijcmas.2018.702.356