National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A total of 100 cattle-calves irrespective of their age, sex and breed brought to Teaching Veterinary Clinical Complex of College of Veterinary and Animal science, Bikaner for treatment were screened for Theileria annulata infection. Blood samples were collected from jugular vein aseptically in vacutainers with and without anticoagulant for genomic DNA and serum isolation, respectively. The entire isolated DNA samples were subjected to nested polymerase chain reaction (nPCR), out of which 41 samples were found positive for Theileria annulata infection. All the serum samples were analyzed by using IDEXX vet test TM kit as per standard procedure. Serum biochemical analyses revealed statistically highly significant (P<0.01) decrease in Mean ± SE values of serum albumin and albumin-globulin ratio while non-significant (P>0.05) decrease in total protein and serum calcium; significant (P<0.05) decrease in serum glucose; significant (P<0.05) increase in serum alkaline phosphatase and SGOT while non-significant (P>0.05) increase in Blood urea nitrogen, serum creatinine, SGPT and serum globulin levels in nPCR positive cattle-calves as compared to nPCR negative cattle-calves.
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