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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 2927-2932
DOI: https://doi.org/10.20546/ijcmas.2018.702.355


Serum Biochemical Study to Cattle-calves naturally infected with Theileria annulata Confirmed by Nested Polymerase Chain Reaction in Bikaner District of Rajasthan, India
Pavan Goyal*, Anju Chahar, R.K. Tanwar, Fakhruddin and S.S. Soni
Department of Epidemiology and Preventive Veterinary Medicine, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Sciences, Bikaner-334 001, Rajasthan, India
*Corresponding author
Abstract:

A total of 100 cattle-calves irrespective of their age, sex and breed brought to Teaching Veterinary Clinical Complex of College of Veterinary and Animal science, Bikaner for treatment were screened for Theileria annulata infection. Blood samples were collected from jugular vein aseptically in vacutainers with and without anticoagulant for genomic DNA and serum isolation, respectively. The entire isolated DNA samples were subjected to nested polymerase chain reaction (nPCR), out of which 41 samples were found positive for Theileria annulata infection. All the serum samples were analyzed by using IDEXX vet test TM kit as per standard procedure. Serum biochemical analyses revealed statistically highly significant (P<0.01) decrease in Mean ± SE values of serum albumin and albumin-globulin ratio while non-significant (P>0.05) decrease in total protein and serum calcium; significant (P<0.05) decrease in serum glucose; significant (P<0.05) increase in serum alkaline phosphatase and SGOT while non-significant (P>0.05) increase in Blood urea nitrogen, serum creatinine, SGPT and serum globulin levels in nPCR positive cattle-calves as compared to nPCR negative cattle-calves.


Keywords: Cattle-calves, Serum, nPCR, SGOT, SGPT, DNA, Theileria annulata, Prevalence
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How to cite this article:

Pavan Goyal, Anju Chahar, R.K. Tanwar, Fakhruddin and Soni, S.S. 2018. Serum Biochemical Study to Cattle-calves naturally infected with Theileria annulata Confirmed by Nested Polymerase Chain Reaction in Bikaner District of Rajasthan.Int.J.Curr.Microbiol.App.Sci. 7(2): 2927-2932. doi: https://doi.org/10.20546/ijcmas.2018.702.355