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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 2921-2926
DOI: https://doi.org/10.20546/ijcmas.2018.702.354


Effect of Natural Compounds on Inhibition of Biofilm Formation of Multi Drug Resistant Staphylococcus aureus and Staphylococcus epidermidis - an in vitro Study
Ankita Agrawal* and Uma Chaudhary
Department of Microbiology, Pt. B. D. Sharma PGIMS, Rohtak, Haryana, India
*Corresponding author
Abstract:

Biofilm formation by Staphylococcus aureus and Staphylococcus epidermidis is a common cause of chronic infection and intravascular device failure. Biofilms are resistant to almost all available drugs rendering them difficult to treat. Thus, there is a need to identify natural compounds which can inhibit biofilm formation. A total of 52 multi drug resistant isolates of Staphylococcus aureus and Staphylococcus epidermidis were isolated from various clinical samples. Isolates were identified by standard microbiological procedures. Biofilm formation was seen by modified tissue culture plate method. The in vitro inhibitory activity of reserpine, eugenol, linoleic acid, curcumin, chitosan and berberine in strong biofilm producing isolates with their minimum biofilm inhibitory concentration (MBIC) was calculated. Biofilm formation was seen in 70.4% isolates of Staphylococcus epidermidis and 48% isolates of Staphylococcus aureus. In isolates of Staphylococcus aureus, the maximum activity for biofilm inhibition was observed with reserpine (MBIC 0.0156 mg/ml) while in case of Staphylococcus epidermidis with eugenol (MBIC 0.0312 mg/ml). In our study, natural compounds were found effective in vitro for inhibition of biofilm formation in Staphylococcus aureus and Staphylococcus epidermidis with reserpine and eugenol most effective and curcumin least effective.


Keywords: Biofilm inhibition, Natural compounds, Minimum biofilm inhibitory concentration, Multi drug resistance, Staphylococci
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How to cite this article:

Ankita Agrawal and Uma Chaudhary. 2018. Effect of Natural Compounds on Inhibition of Biofilm Formation of Multi Drug Resistant Staphylococcus aureus and Staphylococcus epidermidis - an in vitro Study.Int.J.Curr.Microbiol.App.Sci. 7(2): 2921-2926. doi: https://doi.org/10.20546/ijcmas.2018.702.354