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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 2864-2869
DOI: https://doi.org/10.20546/ijcmas.2018.702.349


Detection of Metallo-Beta-Lactamase Production among Klebsiella pneumoniae Isolates by Phenotypic Detection Methods
Rama Sikka, Meenu Garg, Kausalya Raghuraman* and Uma Chaudhary
Department of Microbiology, Pt. B.D. Sharma PGIMS, Rohtak, Haryana, India
*Corresponding author
Abstract:

Resistance to carbapenems is an emerging problem among Klebsiella pneumoniae isolates. A simple and effective phenotypic detection method for carbapenemase production is required in health care settings. A total of 100 clinical isolates of Klebsiella pneumoniae from various samples were screened for imipenem resistance. The imipenem resistant strains were further tested for metallo-beta-lactamase (MBL) production by imipenem combined disc test and ceftazidime combined disc test. Among the 100 isolates, 62 isolates were imipenem resistant and of these 45 were MBL producers. Ceftazidime combined disc method alone could detect 24 (53.3%), imipenem combined disc method alone could detect 12 (26.6%) and nine (20%) of the isolates were positive by both these methods. The present study highlights the burden of MBL producing Klebsiella pneumoniae in a North Indian tertiary care hospital. The study findings suggest that using ceftazidime combined disc test alone had a better yield of MBL when compared to imipenem combined disc test alone. However, combination of both the methods resulted in greater detection of MBL production.


Keywords: Metallo-beta-lactamases, Klebsiella pneumoniae, Ceftazidime combined disc test, Imipenem combined disc test
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How to cite this article:

Rama Sikka, Meenu Garg, Kausalya Raghuraman and Uma Chaudhary. 2018. Detection of Metallo-Beta-Lactamase Production among Klebsiella pneumoniae Isolates by Phenotypic Detection Methods.Int.J.Curr.Microbiol.App.Sci. 7(2): 2864-2869. doi: https://doi.org/10.20546/ijcmas.2018.702.349