National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The search for anticancer drug has lead researchers to study actinomycin D, a chromopeptide antibiotic that destroy cancer cells. The antitumor activity of actinomycin D analyzed on different cell lines by MTTassay. Different concentrations of compound (0.01-1.25µg/mL) was added to cell lines and incubated for 72 hrs. The compound actinomycin D showed cytotoxicity against U251, HCT-116 and MCF-7 cancer cell lines depending on the dose and incubation time. The best activity was shown against U251 cell line with IC50 value of1.07µg/ml on24 hr, 0.56µg/ml on48 hr and 0.028µg/ml on 72hr. The morphological change in actinomycin D treated U251 cells were detected by acridine orange and ethidium bromide staining. The comet assay revealed that actinomycin D inhibited the growth of U251 cells by induction of DNA damage.
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