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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 405-411
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.044


Evaluation of the Efficacy of the Compound Actinomycin D in Tumor Cell Lines
R.Cibi and A. Jayakumaran Nair*
 Department of Biotechnology, University of Kerala, Karyavattom, Trivandrum, India
*Corresponding author
Abstract:

The search for anticancer drug has lead researchers to study actinomycin D, a chromopeptide antibiotic that destroy cancer cells. The antitumor activity of actinomycin D analyzed on different cell lines by MTTassay. Different concentrations of compound (0.01-1.25µg/mL) was added to cell lines and incubated for 72 hrs.  The compound actinomycin D showed cytotoxicity against U251, HCT-116 and MCF-7 cancer cell lines depending on the dose and incubation time. The best activity was shown against U251 cell line with IC50 value of1.07µg/ml on24 hr, 0.56µg/ml on48 hr and 0.028µg/ml on 72hr. The morphological change in actinomycin D treated U251 cells were detected by acridine orange and ethidium bromide staining. The comet assay revealed that actinomycin D inhibited the growth of U251 cells by induction of DNA damage. 


Keywords: Actinomycin D, Cytotoxicity, Acridine orange and ethidium bromide staining, Comet assay
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How to cite this article:

Cibi, R., and Jayakumaran Nair, A. 2016. Evaluation of the Efficacy of the Compound Actinomycin D in Tumor Cell LinesInt.J.Curr.Microbiol.App.Sci. 5(7): 405-411. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.044