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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 2704-2710
DOI: https://doi.org/10.20546/ijcmas.2018.702.328


Determination of Vibrio parahaemolyticus from Retail Shrimp of South Gujarat of Navsari District and their Drug Resistance Pattern
R.K. Patel1, C.V. Savalia1, R. Kumar1*, A.P. Suthar1, S.A. Patel1, N.G. Patel2, I.H. Kalyani3 and S. Gupta2
1Department of Veterinary Public Health and Epidemiology
2Department of Livestock Products Technology
3Department of Veterinary Microbiology, College of Veterinary science & Animal Husbandry, Navsari Agricultural University, Navsari-396 450, Gujarat, India
*Corresponding author
Abstract:

In this study systematic bacteriological examination of aseptically collected 150 shrimp samples, 27 samples of hand swabs from fish handlers were collected from retail fish outlets, and 23 stool samples from patients suffered with digestive disturbances after consumption of seafood reported to private clinics, all from Navsari city were investigated. The samples were enriched in Alkaline Peptone Water followed by direct plating on selective media viz. Thiosulfate Citrate Bile Salt Sucrose agar and Vibrio parahaemolyticus sucrose agar. All samples were brought to the laboratory for isolation, identification and characterization of V. parahaemolyticus isolates on the basis of their morphological, cultural and biochemical characteristics. Out of total 150 samples of shrimp 5 (3.33%) isolates of V. parahaemolyticus were obtained which included 3 (4.28%, 3/70) from marine shrimp and 2 (2.5%, 2/80) from freshwater shrimp samples. Out of 27 samples of the hand swabs of fish handlers, 2 (7.40 %) were positive for V. parahaemolyticus. However, none of 23 human stool samples was positive for the pathogen. All the 7 V. parahaemolyticus isolates were further processed to study drug resistance pattern against 7 commonly used antibiotics by agar disc diffusion method. The V. parahaemolyticus isolates showed cent percent sensitivity towards Ciprofloxacin and Gentamicin, followed in descending order by Amikacin (85.71%), Trimethoprim (85.71%), Streptomycin (71.42%) and Tetracycline (28.58%). An intermediate sensitivity of isolates was noticed against Streptomycin (28.58%) and Amikacin (14.29%). All V. parahaemolyticus isolates were totally resistant to Penicillin. However, varying degree of resistance was observed against Tetracycline (71.42%) and Trimethoprim (14.29%).


Keywords: Shrimp, Vibrio parahaemolyticus, Antibiotic resistance
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How to cite this article:

Patel, R.K., C.V. Savalia, R. Kumar, A.P. Suthar, S.A. Patel, N.G. Patel, I.H. Kalyani and Gupta, S. 2018. Determination of Vibrio parahaemolyticus from Retail Shrimp of South Gujarat of Navsari District and Their Drug Resistance Pattern.Int.J.Curr.Microbiol.App.Sci. 7(2): 2704-2710. doi: https://doi.org/10.20546/ijcmas.2018.702.328