Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 2697-2703
DOI: https://doi.org/10.20546/ijcmas.2018.702.327


Assessment of Different Methods for Sap Transmission of Chilli Leaf Curl Virus in Capsicum annuum L. and Its Validation through PCR Amplification of AV1 Coat Protein Gene
Tanvi Chauhan1*, Amit Choudhary2, Dharmendra Kumar3 and K.N. Singh1
1Department of Plant Molecular Biology and Genetic Engineering, N.D. University of Agriculture and Technology, Kumarganj, Faizabad, India
2National Botanical Research Institute, Lucknow, India
3Department of Plant Pathology, Banda University of Agriculture and Technology,
Banda, India
*Corresponding author
Abstract:

Chilli (Capsicum annuum L.) is an important spice crop severely affected by the virus causes leaf curl symptoms. Efficient transmission of Chilli Leaf Curl Virus was done by using a standardized buffer for sap inoculation to chilli plants. In present investigation, we have used three simple methods i.e. inoculation by syringe, rubbing and by immersion (dip) for sap inoculation and found reproducible when the sap from the infected plants was inoculated onto healthy plants. Out of the three methods used, syringe inoculation was found most efficient method for sap transmission. Syringe inoculation caused most severe symptoms of leaf curl followed by immersion dip after 6 weeks after the inoculation. The sap transmission of chilli leaf curl virus was confirmed by coat protein gene amplification. PCR amplification showed the presence of 320bp single DNA fragment of AV1 coat protein gene that confirmed the presence of Chilli leaf curl virus in the sap transmitted plants.


Keywords: Sap transmission, Gemini virus, Coat protein, Immersion, ToLCD, ChCLV
Download this article as Download

How to cite this article:

Tanvi Chauhan, Amit Choudhary, Dharmendra Kumar and Singh, K.N. 2018. Assessment of Different Methods for Sap Transmission of Chilli Leaf Curl Virus in Capsicum annuum L. and Its Validation through PCR Amplification of AV1 Coat Protein Gene.Int.J.Curr.Microbiol.App.Sci. 7(2): 2697-2703. doi: https://doi.org/10.20546/ijcmas.2018.702.327