National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Macrophage migration inhibitory factor (MIF)is multifunction sproinflammatory cytokine. MIF binds to CD74 which form a complex with CD44 characterized by high affinity for inducing signaling cascade. This study was designed to evaluate the role of MIF in bladder cancer progression. Seventy three biopsy and blood samples have been collected from urinary bladder carcinoma (UBC) patients after cystoscopy surgery and 10 normal bladder biopsies collected from forensic autopsy. MIF level was estimated in seventy three patients with (UBC) at different stages, 32 patients with urinary bladder disorders (UBD) and 30 healthy volunteers by sandwich ELISA. Sections were stained immunohistochemically for CD74and CD44. MIF level was significantly higher in UBC in comparison with UBD and healthy especially in stages T3 and T4 (high grade).Fifty (68.49) % out of 73 UBC gave positivity staining of CD74 and score+3 showed the highest frequency while all normal tissues expressed negative pattern . Moreover, 64(87.7) % of UBC and 9 (90) % normal urothelium gave positive staining results for CD44and score+2represented the highest frequency among scores. Serum level of MIF is significantly elevated with higher grade of advanced stage than low grade of primary stage .Over expression of MIF with higher expression of CD74 and CD44 may promote malignant cell transformation in urinary bladder carcinoma.
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