Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 2606-2610
DOI: https://doi.org/10.20546/ijcmas.2018.702.317


Effects of Coumestrol Treatment on Spermatogenesis in Dogs
Rajesh Kumar1, A.K. Sharma2, Gyan Singh3* and Ravi Dutt2
1ICAR-Central Institute for Research on Buffaloes, Hisar-125001, Haryana, India
2Department of Veterinary Gynaecology and Obstetrics, LUVAS, Hisar - 125 004, Haryana, India
3Veterinary Clinical Complex, LUVAS, Hisar -125 004, Haryana, India
*Corresponding author
Abstract:

The establishment of safe and effective fertility inhibitors for dogs must also be matched by evidence that non-surgical fertility control is an efficient, humane, and socially acceptable alternative to surgical sterilization for managing over abundant populations of dogs and their impact on human interests. The present study was undertaken to study the contraceptive effects of coumestrol in adult male dogs. Experimental group I was given 300 µg coumestrol dissolved in di-methyl sulfoxide orally in the commercial dog treats, on days 0, 7, 14, 21 and 28. Control group II was given DMSO alone as per above schedule. One week after the end of treatment period, dogs were anaesthetized with xylazine (2.0mg/kg B. weight) and ketamine HCl (10mg/kg B. weight). Testicles were removed and efferent ductules were collected and fixed in Bouin’s fixatives. Tissues were dehydrated in methanol and cleared in xylene, and then paraffin blocks were prepared. Sections were cut at 5-7 µ and stained with Harris-hematoxylin and Eosin. Histological examination of the testicular tissues revealed normal germinal epithelium. Therefore, it was concluded that oral feeding of 300 µg coumestrol did not have any adverse effect on male dog fertility.


Keywords: Coumestrol, Dogs, Histology, Spermatogenesis, Sterilization
Download this article as Download

How to cite this article:

Rajesh Kumar, A.K. Sharma, Gyan Singh and Ravi Dutt. 2018. Effects of Coumestrol Treatment on Spermatogenesis in Dogs.Int.J.Curr.Microbiol.App.Sci. 7(2): 2606-2610. doi: https://doi.org/10.20546/ijcmas.2018.702.317