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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 364-371
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.039


Comparison of Antimicrobial Susceptibility Pattern of Biofilm Producing and Nonbiofilm Producing Staphylococci Isolated from Various Clinical Samples of Indoor Patients
Vijay Laxmi* and Rajni Sharma
 Department of Microbiology, SMS Medical College and Attached Hospitals,Jaipur, Rajasthan, India
*Corresponding author
Abstract:

Biofilms are group of microorganisms encased in an exopolymeric coat. They have been associated with a variety of persistent infections that respond poorly to conventional antibiotics. To evaluate three different methods for detection of biofilm formation in staphylococci. For detection of biofilm formation, 120 clinical isolates of staphylococcus spp. were screened by Congo red agar (CRA) methods, Tube methods (TM) and Tissue culture plate (TCP) methods.  Out of 120 Staphylococcus spp., 72 were coagulase positive staphylococci (CPS)  and 48 were coagulase negative staphylococci (CNS). 59.72% of CPS and 58.33% of CNS were slime producers. 71 isolates were detected as slime producer by TCP method, 56 by TM and 34 by CRA method. High resistances to conventional antibiotics were shown by biofilm producers. The TCP method was found to be most sensitive, accurate and reproducible screening method for detection of biofilm formation by staphylococci and has advantage of being a quantitative model to study the adherence of staphylococci on bio-medical devices.


Keywords: Biofilm detection, Staphylococcus, tissue culture plate (TCP), Congo red agar (CRA), Tube method (TM).  
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How to cite this article:

Vijay Laxmi and Rajni Sharma. 2016. Comparison of Antimicrobial Susceptibility Pattern of Biofilm Producing and Nonbiofilm Producing Staphylococci Isolated from Various Clinical Samples of Indoor Patients.Int.J.Curr.Microbiol.App.Sci. 5(7): 364-371. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.039