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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 2125-2134
DOI: https://doi.org/10.20546/ijcmas.2018.702.254


Incidence of Hepatopancreatic Microsporidiasis, by Enterocytozoon hepatopenaei (EHP) in Penaeus vannamei Culture in Nellore District, Andhra Pradesh, India and the Role of Management in its Prevention and Transmission
Suresh Kummari1, Divya V. Haridas2, Sevok Handique3, Sam Peter2, C.G. Rakesh2, K.G. Sneha2, B. Manojkumar4 and Devika Pillai2*
1Department of Aquatic Animal Health Management, College of Fishery Science, Pebbair, P.V. Narsimha Rao Veterinary University, Hyderabad, India
2Department of Aquatic Animal Health Management, Kerala University of Fisheries and Ocean Studies, Panangad, Cochin, India
3Division of Fisheries, ICAR Research Complex for NEH Region, Umiam, Meghalaya, India
4 Department of Fishery Engineering, Kerala University of Fisheries and Ocean Studies, Panangad, Cochin, India
*Corresponding author
Abstract:

Shrimp culture is the most lucrative aquaculture sector in Asia-Pacific region. Recently, shrimp farms in India have been affected by the microsporidian parasite, Enterocytozoon hepatopenaei (EHP) associated with retarded growth without other clinical signs, causing significant economic losses to the shrimp industry. The present study was carried out to investigate the incidence of EHP in Penaeus vannamei farms of Nellore district of Andhra Pradesh, and to identify the carriers of EHP and the role of management in reducing the incidence of EHP in ponds. PCR analysis by two sets of EHP specific primers indicated the incidence of EHP to be 92.50%. Histopathological studies of the hepatopancreas of affected shrimp showed sloughing of the epithelial cells of hepatopancreatic tubules, lifting and detachment of epithelial layers of hepatopancreatic tubules and heavy damage of hepatopancreas. PCR screening of aquatic macro fauna in the farms showed that samples of crabs, polychaetesand non-penaeids were positive for EHP, implying that they could act as carriers of EHP. On the other hand, fishes were found negative. Strict implementation of Best Management Practices (BMPs) in one selected farm which was confirmed to be infected by EHP clearly showed that practice of BMPs and avoidance of carriers can definitely reduce the incidence of EHP during the subsequent culture in shrimp farms. After implementation of BMPs, significant improvement in growth rate, and Feed Conversion Ratio (FCR) were also observed. In the control pond where BMPs were not implemented, EHP continued to be present, affecting growth and FCR adversely. This is the first report comparing the role of management in EHP infection and identifying carriers of EHP in P. vannamei culture in India.


Keywords: Enterocytozoon hepatopenaei, Histopathology, PCR, Microsporidian parasite, India
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How to cite this article:

Suresh Kummari, Divya V. Haridas, Sevok Handique, Sam Peter, C.G. Rakesh, K.G. Sneha, B. Manojkumar and Devika Pillai 2018. Incidence of Hepatopancreatic Microsporidiasis, by Enterocytozoon hepatopenaei (EHP) in Penaeus vannamei Culture in Nellore District, Andhra Pradesh, India and the Role of Management in its Prevention and Transmission.Int.J.Curr.Microbiol.App.Sci. 7(2): 2125-2134. doi: https://doi.org/10.20546/ijcmas.2018.702.254