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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 1674-1691
DOI: https://doi.org/10.20546/ijcmas.2018.702.203


Isolation and Characterization of Methanol Utilizing Microorganisms from Leguminous Crops
Umang Ahlawat, Rekha Sansanwal, Priyanka Batra and Leela Wati*
Department of Microbiology, College of Basic Science and Humanities, CCS HAU Haryana Agricultural University, Hisar, Haryana, India
*Corresponding author
Abstract:

A total of sixty isolates were obtained from rhizosphere, nodule and phyllosphere of different crops. These isolates were analysed for some of their plant growth promoting traits like IAA production, phosphate solubilisation, siderophore production, HCN production, ACC utilization and ammonia excretion. Apart from these traits all the isolates were also screened for their growth at different temperatures and salt concentrations. Three promising isolates (MP12, PP1 and UP4) were characterized morphologically, biochemically and on the basis of 16S rRNA gene sequencing. Phylogenetic tree of methanol utilizing isolates MP12, PP1 and UP4 was prepared by using neighbour joining of BLAST programme. The isolate MP12 showed 98% similarity with Methylobacterium oryzae, isolate PP1 showed 99% similarity with Ochrobacterium intermedium and isolate UP4 showed 95% similarity with Microbacterium ginsengisoli. All these three isolates were able to produce IAA, siderophore, ACC and other plant growth promoting traits. Therefore, these results suggest the possibility to use these methanol utilizing bacteria as biological fertilizers to increase pulse crop production.


Keywords: Biofertilizer, Methanol, Nodule, Rhizosphere, Phylloshpere
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How to cite this article:

Umang Ahlawat, Rekha Sansanwal, Priyanka Batra and Leela Wati. 2018. Isolation and Characterization of Methanol Utilizing Microorganisms from Leguminous Crops.Int.J.Curr.Microbiol.App.Sci. 7(2): 1674-1691. doi: https://doi.org/10.20546/ijcmas.2018.702.203