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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 267-274
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.027


Speciation of Candida using HiCrome Candida Differential Agar
Soumya Kaup1*, Jaya Sankarankutty2, H.V. Balasubrahmanya3, Suma Kulkarni1 and M. Nirmala1
1Department of Microbiology, Shridevi Institute of Medical Sciences and Research Hospital, Sira Road, NH-04, Tumkur-572106, India
2Department of Hospital Infection Control, Narayana Hrudayalaya Hospital, Bangalore, India
 3P.C. Institute of Paramedical Sciences, Bangalore, India
*Corresponding author
Abstract:

Non-albicans Candida (NAC) species are emerging as important opportunistic pathogens. This has significant clinical impact as, NAC species have decreased susceptibility to commonly used antifungal agents. Hence species identification in the Clinical Microbiology laboratory is essential. This study was conducted to assess efficacy of HiCrome agar to reliably identify Candida to the species level. Altogether 48 isolates of Candida were isolated during a period of one year constituting 24 isolates of Candida albicans, 13 of Candida tropicalis, 4 of Candida krusei, 4 of Candida glabrata, 2 of Candida parapsilosis and 1 isolate of Candida guillermondii. Identification was done based on microscopic morphology, germ tube test, growth at 45°C, morphology on corn meal agar and colony colour on HiCrome agar. HiCrome agar accurately identified all species of Candida albicans, Candida tropicalis, Candida krusei and Candida glabrata. Two isolates of Candida parapsilosis and one isolate of Candida guillermondii was misidentified as Candida glabrata. HiCrome agar can be used as a fairly reliable and time saving alternative to conventional methods with good sensitivity and specificity.


Keywords: Candida,HiCrome agar, Cornmeal agar, chromogenic media.
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How to cite this article:

Soumya Kaup, Jaya Sankarankutty, H.V. Balasubrahmanya, Suma Kulkarni and M. Nirmala. 2016. Speciation of Candida using HiCrome Candida Differential Agar.Int.J.Curr.Microbiol.App.Sci. 5(7): 267-274. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.027