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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 1194-1205
DOI: https://doi.org/10.20546/ijcmas.2018.702.147


Biocides Resistance Profiles of Biofilm Forming Bacteria of Dairy Niche and their Control
Mitali Makwana, Chand Ram Grover and Narendra Kumar
1Dairy Microbiology Division, Anand Agricultural University, Anand-388110, Gujarat, India
2Dairy Microbiology Division, National Dairy Research Institute, Karnal-132001, Haryana, India
*Corresponding author
Abstract:

Microbial biofilms are of special concern to dairy and food industry, as biofilms on raw materials or food contact surfaces represent possible sources of contamination with spoilage or pathogenic microbes. The present study was carried out to evaluate biocide resistance profiles of biofilm forming bacteria of dairy niche towards commonly used biocides and their control in biofilm formation. Genetically confirmed 17 isolates of genera Bacillus, Staphylococcus, Salmonella, Pseudomonas, Listeria and E. coli were evaluated for their biocide resistance profiles by determining their minimum inhibitory concentration (MIC) and also microbial biofilm eradicating concentration (MBEC) against commonly used biocides (iodophore, sodium hypochlorite, and benzalkonium chloride) in dairy/food industry. The Listeria, Salmonella and Staphylococcus exhibited highest MBEC against iodophore (550 ppm), Listeria against sodium hypochlorite (550 ppm); Bacillus, Pseudomonas, Listeria and consortia against benzalkonium chloride (650 ppm). Benzalkonium chloride and sodium hypochlorite were found most efficacious against all tested isolates and their consortia as they could reduce higher log counts after treatment in comparison to iodophore.


Keywords: Biofilm, Irreversible, Efficiency, Resistance, Persistence
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How to cite this article:

Mitali Makwana, Chand Ram Grover and Narendra Kumar. 2018. Biocides Resistance Profiles of Biofilm Forming Bacteria of Dairy Niche and their Control.Int.J.Curr.Microbiol.App.Sci. 7(2): 1194-1205. doi: https://doi.org/10.20546/ijcmas.2018.702.147