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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 210-223
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.022


Effect of Sanitizers and Glucose on Clostridium perfringens Biofilm Formation and Growth
Castro Melina Gabriela1, Icazatti Alejandro2, Divizia Julia2, Vega Alba Edith2, Cortiñas Teresa Ines2 and Stagnitta Patricia Virginia2*
 1Laboratory of Microbiology, University of San Luis, Ejercito de Los Andes 950 Bloque I, San Luis, Argentina
2Ejército de Los Andes 950 Bloque I Laboratory of Molecular Biology, IMIBIO,National University of San Luis, Argentina
*Corresponding author
Abstract:

Clostridium perfringens enterotoxigenic strain is one of the responsible of food poisoning; this is caused by cpe enterotoxin. Cpe is produced during sporulation, a resistance mechanism. It is described that C. perfringens can form biofilm under stressed environments and this depends of carbohydrates availability. The aim of this work was to evaluate the ability of enteroxigenic (E+) and no enterotoxigenic (E-) C. perfringens strains to form biofilm at different concentrations of carbon source and the inhibitory effect of various Poli P over biofilm formation and the expression of cpe and ccpa genes. Our results indicate that E+ C. perfringens biofilm reach the more complex structure with high glucose concentrations. Even more current sanitizers used in food industry like SAPP and TSPP, showed to stimulate biofilm formation and to inhibit the growth of planktonic cells. On the other hand, morphological changes were observed during biofilm formation under low glucose concentration and in presence of sanitizers. We propose that the change from a bacillary to coccoid form is related to a resistance to stressed environments. Finally the cpe expression is not related to biofilm formation.


Keywords: Biofilm,C, perfringens, sanitizers,glucose, morphology, CcpA, cpe.
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How to cite this article:

Castro Melina Gabriela, Icazatti Alejandro, Divizia Julia, Vega Alba Edith, Cortiñas Teresa Ines and Stagnitta Patricia Virginia. 2016. Effect of Sanitizers and Glucose on Clostridium perfringens Biofilm Formation and Growth.Int.J.Curr.Microbiol.App.Sci. 5(7): 210-223. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.022