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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 867-872
DOI: https://doi.org/10.20546/ijcmas.2018.702.109


Detection and Molecular Characterization of Methicillin-Resistant Staphylococcus aureus Obtained from Poultry and Poultry House Environment of Anand District, Gujarat, India
K.R. Bhedi1*, J.B. Nayak1, M.N. Brahmbhatt1, A. Roy2, R.A. Mathakiya2 and R.M. Rajpura3
1Department of Veterinary Public Health and Epidemiology
2Department of Veterinary Microbiology, Veterinary College, Anand Agricultural University, Anand, Gujarat, India
3Department of Animal Science, BACA, Anand Agricultural University, Anand, Gujarat, India
*Corresponding author
Abstract:

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the important emerging pathogen worldwide responsible for causing wide range of disease in human and animals. It is responsible for causing nosocomial, community infection but it has been reported to cause livestock infection also. The main aim of the study was to investigate the occurrence of MRSA in raw poultry meat sold at in and around Anand City, Gujarat. A total 23 S. aureus isolates obtained from 250 poultry and poultry house environment samples were taken in this study. All the 23 isolates were confirmed as S. aureus by PCR. Among 23 isolates, four (1.6%) isolates yield positive growth on MeReSa agar giving characteristics appearance of light pink colour colony indicating MRSA positive strain. All these 4 isolates were found positive for mecA gene by PCR which is responsible for Methicillin resistance in S. aureus. The presence of MRSA in poultry meat is of great concern for public health importance.


Keywords: S. aureus, MRSA, Poultry meat, Antibiotic Sensitivity, mecA gene
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How to cite this article:

Bhedi, K.R., J.B. Nayak, M.N. Brahmbhatt, A. Roy, R.A. Mathakiya and Rajpura, R.M. 2018. Detection and Molecular Characterization of Methicillin-Resistant Staphylococcus aureus Obtained from Poultry and Poultry House Environment of Anand District, Gujarat, India.Int.J.Curr.Microbiol.App.Sci. 7(2): 867-872. doi: https://doi.org/10.20546/ijcmas.2018.702.109