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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 836-840
DOI: https://doi.org/10.20546/ijcmas.2018.702.105


Isolation of Staphylococcus aureus from Raw Cattle Milk and their Drug Resistance Pattern
R.K. Patel1, R. Kumar1*, C.V. Savalia1 and N.G. Patel2
1Department of Veterinary Public Health and Epidemiology, College of Veterinary science & Animal Husbandry, Navsari Agricultural University, Navsari-396 450, Gujarat, India
2Department of Livestock Products Technology, College of Veterinary science & Animal Husbandry, Navsari Agricultural University, Navsari-396 450, Gujarat, India
*Corresponding author
Abstract:

Staphylococcus aureus is one of the important human pathogens involved in food related diseases and common community associated infections. This organism proliferates in food and causes food-borne illnesses. Milk serves as an ideal medium for growth of many microorganisms including Staphylococcus aureus. A total of 118 raw cattle milk samples were collected under aseptic precautions from different places of Navsari district of south Gujarat, processed under standard bacteriological techniques. The Baird Parker Agar was sued as selective medium for isolation. The presumptive isolates were identified on the basis of their morphological, cultural and biochemical characteristics. The sensitivity pattern of S. aureus with different antimicrobial agents was evaluated by disk diffusion method. Analysis of result revealed 12 isolates (10.16 %) of S. aureus from 118 milk samples. The S. aureus isolates showed cent percent sensitivity towards Amikacin and Gentamicin, followed in reducing levels by Gatifloxacin (91.66 %), Ciprofloxacin (91.66 %), 75 % each, of Streptomycin and Kanamycin; Ampicillin (66.66 %) and Cephalexin (41.66 %). The pattern clearly indicated overall high percent resistance to Cephalexin (58.33%), followed by Ampicillin (33.33 %), Methicillin (25.00%), Kanamycin (16.66 %) and Gatifloxacin (8.33 %), Ciprofloxacin (8.33 %). The isolates showed intermediate sensitivity towards Streptomycin (25.00 %) and Kanamycin (8.33 %). Findings of the study suggested judicious use of antimicrobial therapy in milking animals.


Keywords: Antibiotics, Food, Raw milk, S. aureus, Food poisoning
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How to cite this article:

Patel, R.K., R. Kumar, C.V. Savalia and Patel, N.G. 2018. Isolation of Staphylococcus aureus from Raw Cattle Milk and Their Drug Resistance Pattern.Int.J.Curr.Microbiol.App.Sci. 7(2): 836-840. doi: https://doi.org/10.20546/ijcmas.2018.702.105