National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The enzyme invertase is produced by Saccharomyces cerevisiae. It hydrolyzes sucrose into glucose and fructose, having a great utility industrially. With this it is necessary to know its main characteristics to be able to know how to optimize it. In this research the enzyme invertase was worked in two types of reactors very common in the industrial area, the batch reactor and the fixed bed reactor. In the experiments described here, the type of reaction involved in hydrolysis, whether exothermic or endothermic, and what is its activation energy was determined. In the characterization of the enzyme the half-life and its maximum yield were determined. The DNS methodology was applied and a spectrophotometer of the FEMTO brand was used. The results obtained here show that the hydrolysis of saccharose is an exothermic reaction with activation energy of 25.07 kJ. The invertase enzyme used herein has a half-life of 65.39 min and a maximum yield of 47.02%. All adjusted curves had a R² value above 0.91, proving the reliability of the obtained results.
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