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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 806-816
DOI: https://doi.org/10.20546/ijcmas.2018.702.102


Activation Energy, Half-Life and Yield of the Hydrolysis Reaction of Sucrose Catalyzed by the Enzyme Invertase Produced by Yeast Saccharomyces cerevisiae
Erich Potrich1* and Larissa Souza Amaral2
1Department of Chemical Engineering PPGEQ, Federal University of São Carlos – UFSCar. São Carlos - SP, 13566-590, Brazil
2Program Interunits in Bioengineering EESC/FMRP/IQSC, University of São Paulo – USP. São Carlos - SP, 13566-590, Brazil
*Corresponding author
Abstract:

The enzyme invertase is produced by Saccharomyces cerevisiae. It hydrolyzes sucrose into glucose and fructose, having a great utility industrially. With this it is necessary to know its main characteristics to be able to know how to optimize it. In this research the enzyme invertase was worked in two types of reactors very common in the industrial area, the batch reactor and the fixed bed reactor. In the experiments described here, the type of reaction involved in hydrolysis, whether exothermic or endothermic, and what is its activation energy was determined. In the characterization of the enzyme the half-life and its maximum yield were determined. The DNS methodology was applied and a spectrophotometer of the FEMTO brand was used. The results obtained here show that the hydrolysis of saccharose is an exothermic reaction with activation energy of 25.07 kJ. The invertase enzyme used herein has a half-life of 65.39 min and a maximum yield of 47.02%. All adjusted curves had a R² value above 0.91, proving the reliability of the obtained results.


Keywords: Enzyme invertase, Sucrose hydrolysis, DNS method, Activation energy, Half-life, Yield
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How to cite this article:

Erich Potrich and Larissa Souza Amaral. 2018. Activation Energy, Half-Life and Yield of the Hydrolysis Reaction of Sucrose Catalyzed by the Enzyme Invertase Produced by Yeast Saccharomyces cerevisiae.Int.J.Curr.Microbiol.App.Sci. 7(2): 806-816. doi: https://doi.org/10.20546/ijcmas.2018.702.102