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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 795-805
DOI: https://doi.org/10.20546/ijcmas.2018.702.101


Cell Membrane Damage of Prokaryotes and Analysis of the Cell Death Type in Eukaryotes by Fluorescence Microscopy
Larissa Souza Amaral1* and Erich Potrich2
1Program Interunits in Bioengineering EESC/FMRP/IQSC, University of São Paulo – USP. São Carlos - SP, 13566-590, Brazil
2Department of Chemical Engineering PPGEQ, Federal University of São Carlos – UFSCar. São Carlos - SP, 13566-590, Brazil
*Corresponding author
Abstract:

Fluorescence microscopy allows an extensive variety of analyzes in structural biology. Until the beginning of the seventeenth century living beings were limited to macroscopic organisms only, and there was no knowledge of the microscopic universe. Epi-fluorescence and Confocal microscopy appears in 1995 by Marvin Minsky with the aim of increasing the contrast and constructing three-dimensional images by the use of an aperture, allowing a great definition. The use of this microscopic technique is indicated for studies of thin or thick materials, live or pre-fixed and with or without staining by fluorophore molecules. In this study we present the applicability of fluorescence microscopy to identify damage in the cytoplasmic membrane of prokaryotes and mechanisms of cell death in eukaryotes.


Keywords: Fluorescence microscopy, Cell membrane damage, Cell death type, Prokaryotes, Eukaryotes
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How to cite this article:

Larissa Souza Amaral and Erich Potrich. 2018. Cell Membrane Damage of Prokaryotes and Analysis of the Cell Death Type in Eukaryotes by Fluorescence Microscopy.Int.J.Curr.Microbiol.App.Sci. 7(2): 795-805. doi: https://doi.org/10.20546/ijcmas.2018.702.101