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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 639-651
DOI: https://doi.org/10.20546/ijcmas.2018.702.079


Molecular Genetic Diversity and Population Structure Analysis in Chickpea (Cicer arietinum L.) Germplasm using SSR Markers
S.M. Samyuktha, J.R. Kannan Bapu and S. Geethanjali*
Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore- 641 003, Tamil Nadu, India
*Corresponding author
Abstract:

The genetic diversity and population structure in 51 chickpea accessions were studied using 30 chickpea specific SSR markers. Twenty eight SSR markers exhibited polymorphism producing a total of 217 alleles. The average number of alleles per locus was 7.75. The average PIC value was 0.75 and ranged from a minimum of 0.53 (TA1) to a maximum of 0.85 (TA64). The markers TA5, TA14, TA18, TA21, TA64, TA71, TA106, TR20, TR26, TR58, TS43 were considered to be highly informative (PIC ≥ 0.8), in evaluating allelic variation present in the chickpea accessions. Genetic diversity analysis resulted in the formation of two major clusters, using WARD’s method of hierarchical clustering based on the dissimilarity index values. Cluster I represented a heterogeneous group with 42 genotypes representing no affiliation with the geographic regions and cluster II comprised of nine genotypes. Population structure based on allele frequency using Bayesian clustering approach identified discrete subpopulation which was similar to the dendrogram obtained using molecular data. Two groups were obtained (K=2) with the mean FST values of 0.3515 and 0.0972 respectively. However, four accessions were categorized as having admixed ancestry. The results revealed greater resolving power of SSR markers for chickpea germplasm. The availability of wide diversity in the germplasm could be effectively utilized in genetic resource conservation, association mapping as well as in breeding programmes for widening the genetic base of the cultivated chickpea.


Keywords: Chickpea, SSR markers, Genetic diversity, Population structure
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How to cite this article:

Samyuktha, S.M., J.R. Kannan Bapu and Geethanjali, S. 2018. Molecular Genetic Diversity and Population Structure Analysis in Chickpea (Cicer arietinum L.) Germplasm using SSR Markers.Int.J.Curr.Microbiol.App.Sci. 7(2): 639-651. doi: https://doi.org/10.20546/ijcmas.2018.702.079