National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The genetic diversity and population structure in 51 chickpea accessions were studied using 30 chickpea specific SSR markers. Twenty eight SSR markers exhibited polymorphism producing a total of 217 alleles. The average number of alleles per locus was 7.75. The average PIC value was 0.75 and ranged from a minimum of 0.53 (TA1) to a maximum of 0.85 (TA64). The markers TA5, TA14, TA18, TA21, TA64, TA71, TA106, TR20, TR26, TR58, TS43 were considered to be highly informative (PIC ≥ 0.8), in evaluating allelic variation present in the chickpea accessions. Genetic diversity analysis resulted in the formation of two major clusters, using WARD’s method of hierarchical clustering based on the dissimilarity index values. Cluster I represented a heterogeneous group with 42 genotypes representing no affiliation with the geographic regions and cluster II comprised of nine genotypes. Population structure based on allele frequency using Bayesian clustering approach identified discrete subpopulation which was similar to the dendrogram obtained using molecular data. Two groups were obtained (K=2) with the mean FST values of 0.3515 and 0.0972 respectively. However, four accessions were categorized as having admixed ancestry. The results revealed greater resolving power of SSR markers for chickpea germplasm. The availability of wide diversity in the germplasm could be effectively utilized in genetic resource conservation, association mapping as well as in breeding programmes for widening the genetic base of the cultivated chickpea.
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