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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 500-505
DOI: https://doi.org/10.20546/ijcmas.2018.702.063


Characterization of Antimicrobial Peptide Produced by Probiotic Lactobacillus plantarum DM 69 Isolated from Yogurt Sample of Odisha, India
Debapriya Mohanty* and Pratima Ray
Department of Microbiology, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha, India
*Corresponding author
Abstract:

The aim of this study was to characterize the antimicrobial peptide (bacteriocin) produced by a probiotic Lactobacillus plantarum strain DM 69 isolated from yogurt sample of Odisha. Total DNA of L. plantarum DM 69 was extracted and tested for the presence of bacteriocin genes. PCR-amplified DNA showed a single band in gel which partially confirmed the presence of Plantaricin gene responsible for showing antimicrobial activity. The purified antimicrobial peptide was found to be stable at 60 0C, 80 0C up to 60 min and 100 0C for 30 min, pH 2-8, 1-8% NaCl and in presence of digestive enzymes such as α-Amylase, trypsin, lipase and lysozyme.


Keywords: Lactobacillus plantarum, Plantaricin gene, PCR-amplification, Stability of bacteriocin
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How to cite this article:

Debapriya Mohanty and Pratima Ray. 2018. Characterization of Antimicrobial Peptide Produced by Probiotic Lactobacillus plantarum DM 69 Isolated from Yogurt Sample of Odisha.Int.J.Curr.Microbiol.App.Sci. 7(2): 500-505. doi: https://doi.org/10.20546/ijcmas.2018.702.063