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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 171-179
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.017


Genetic Diversity Study of Bacteria Associated with Clarias gariepinus from Ebute-Ibooro on Yewa River using Random Amplified Polymorphic DNA
O.O. Oyelakin1, J.K. Ekelemu2*, A.A. Akinyemi3, A.R. Oloyede1 and M.E. Ige3
1Biotechnology Centre, Federal University of Agriculture, Abeokuta, Nigeria
2Department of Fisheries, Delta State University, Asaba Campus, Delta State, Nigeria
3Department of Aquaculture and Fisheries Management, Federal University of Agriculture, Abeokuta, Nigeria
*Corresponding author
Abstract:

Fish is a relatively inexpensive source of protein and an important source of foreign exchange in many regions of world and water is the physical support in which they carry out their life functions. Genetic study of bacterial isolates found in the guts, gills and skin samples of catfish obtained from EbuteIbooro landing site of Yewa River was carried out using Random Amplified Polymorphic DNA – Polymerase Chain Reaction Techniques. Morphometrics, weight, length of the fishes were recorded and water quality parameters of the water body were also recorded. Bacterial isolates were obtained from samples grown in culture medium using Nutrient agar. Subculturing was done in order to obtain the pure isolates. Five RAPD primers were used, a total of number of 62 markers were generated with 54 being polymorphic and 8 being monomorphic. Two main groups were observed from the dendrogram derived from the RAPD results. Group 1 consists of 1 organism while group 2 consists of 19 organisms. Group 2 were further divided into sub groups with each sub group having its own constituent of organisms. Haemolysis test carried out on this bacteria isolates to determine their pathogenicity showed that 12.5% of the organisms indicated complete haemolysis, 37.5% indicated incomplete haemolysis while the remaining 50% indicated no haemolysis. The results show that RAPD fingerprinting is one of the best molecular tools for identification.


Keywords: Clarias gariepinus, Bacteria,RAPD markers, Haemolysis.
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How to cite this article:

Oyelakin, O.O., J.K. Ekelemu, A.A. Akinyemi, A.R. Oloyede and Ige, M.E. 2016. Genetic Diversity Study of Bacteria Associated with Clarias gariepinus from Ebute-Ibooro on Yewa River using Random Amplified Polymorphic DNAInt.J.Curr.Microbiol.App.Sci. 5(7): 171-179. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.017