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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 486-493
DOI: https://doi.org/10.20546/ijcmas.2018.702.061


Biofilm Production by Organisms Causing Ventilator Associated Pneumonia
P. Nanthini Devi1* and S. Gomathi2
1Department of Microbiology, Sree Balaji Medical College and Hospital, Chennai, India
2Department of Microbiology, SRM Medical College Hospital and Research centre, Kattankulathur, India
*Corresponding author
Abstract:

Ventilator-associated pneumonia is defined as nosocomial pneumonia occurring more than 48 hours after patients have received mechanical ventilation. VAP in a critically ill patient significantly increases risk of mortality, ventilator time, length of stay and cost of care. The Endotracheal tube is a favourable site for biofilm formation. They are adherent cells which are embedded within a self-produced matrix of This EPS matrix impedes the normal functions of antibodies and the phagocytic cells of the immune system and leads to increased resistance to antibiotics. Aim of this study is to isolate and identify the organisms causing VAP and demonstrate biofilm production. A total of 149 patients were selected for the study. 40.94% developed VAP. The organisms causing VAP was isolated and identified by conventional tests. The organisms were tested for biofilm production by the microtitre plate method using safranin dye and their optical densities measured. The organisms causing VAP were Acinetobacter baumannii, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus. 47.73 % were strong biofilm producers, 28.41% were moderate producers, 21.59% were weak producers and 2.27% did not produce any biofilm. Among the 42 isolated which produced strong biofilm, Acinetobacter baumannii was the most common organism associated with strong biofilm formation (64.29%) followed by Klebsiella pneumoniae (21.43%). The biofilm production by microorganisms is a threat because of its resistance to antimicrobial drugs. This highlights the importance of discovering new strategies focused in the removal of biofilm from the Endotracheal tube (ETT).Well planned and early extubations should be done to prevent biofilm formation on the ETT.


Keywords: Ventilator associated pneumonia, Biofilms, tissue culture plate method, Multidrug resistance
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How to cite this article:

Nanthini Devi, P. and Gomathi, S. 2018. Biofilm Production by Organisms Causing Ventilator Associated Pneumonia.Int.J.Curr.Microbiol.App.Sci. 7(2): 486-493. doi: https://doi.org/10.20546/ijcmas.2018.702.061