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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 429-445
DOI: https://doi.org/10.20546/ijcmas.2018.702.055


Role of Aspergillus flavus on Biodegradation of Lignocellulosic Waste Millet Straw and Optimization Parameters for Enzyme Hydrolysis and Ethanol Production under Solid State Fermentation
Prathvi V. Narsale, Sejal R. Patel* and Pradeep Acharya
M. D. Gram Seva Mahavidyalaya, Gujarat Vidyapeeth, Sadra, Gandhinagar, India
*Corresponding author
Abstract:

Amongst six natural lignocellulosic waste/raw material screened, millet straw contained maximum cellulose (30 %) and physical and chemical pre-treatment yielded maximum cellulase activity. The composition of millet straw showed 30 % cellulose, 10 % hemicellulose, 8.73 % total organic carbon, 0.7 % total nitrogen and 0.8 ppm phosphorous. Amongst four fungi of genus Aspergillus isolated from local soil dumps of kitchen and garden waste, Aspergillus flavus showed maximum cellulase activity on CMC agar medium and used for degradation of millet straw. Amongst five media, Mandle and Sternberg fermentation medium showed maximum enzyme activity due to favorable pH and supplementing nitrogen and mineral requirements for growth and development of Aspergillus flavus. The optimum parameters for maximum cellulolytic enzyme activity in solid state fermentation were also studied which showed that 1 ml inoculum size, 4.5 pH of the medium, incubation at 28 °C and 70 % moisture content for 120 h, 3.0 % w/v substrate concentration, 0.1% supplementation of carbon source in form of Carboxy Methyl Cellulose and 0.5 % of nitrogen source in form of ammonium sulphate found effective for better endoglucanase activity. The hydrolysate was subjected to saccharification (fermentation through yeasts Saccharomyces cerevisiae) having potential of producing 0.15 g/hour/liter of ethanol at above optimized parameters.


Keywords: Lignocellulosics waste, Millet straw, Cellulase, Aspergillus flavus, CMC agar medium, Solid state fermentation, Endoglucanase, Saccharification, Saccharomyces cerevisiae
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How to cite this article:

Prathvi V. Narsale, Sejal R. Patel and Pradeep Acharya. 2018. Role of Aspergillus flavus on Biodegradation of Lignocellulosic Waste Millet Straw and Optimization Parameters for Enzyme Hydrolysis and Ethanol Production under Solid State Fermentation.Int.J.Curr.Microbiol.App.Sci. 7(2): 429-445. doi: https://doi.org/10.20546/ijcmas.2018.702.055