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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 153-163
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.015


Isolation, Identification and Characterization of Keratinolytic Streptomyces coelicoflavus
R.S. Jadhav1*, D.D. Karad2 and S.W. Kulakrni3
1Department of Microbiology, Vishwasrao Naik Art’s, Commerce and Baba Naik Science Mahavidyalaya, Shirala 415408, Dist-Sangli (M.S.) India.
2Research Department of Microbiology, Shriman Bhausaheb Zadbuke Mahavidyalaya, Barshi 413 401, Dist-Solapur(M.S.) India.
3Research Department of Microbiology, Shri Shivaji Mahavidyalaya, Barshi 413 401,Dist-Solapur (M.S.) India
*Corresponding author
Abstract:

Fourteen isolates were obtained from poultry and beam house region soils of Sangli district. An isolate showing maximum keratinolysis was identified as Streptomyces coelicoflavus. The ability of Streptomyces coelicoflavus to utilize chicken feather as a substrate was tested. Crude extract of keratinase enzyme from feather meal broth was used for characterization. It was found that maximum enzyme activitywas49U/ml. Crude keratinase was optimally active in the pH range from 7.5 to 8 and at temperatures from 350C to400C. The various divalent metal ion were tested, Ca++ and Zn++ were found to enhance the activity whereas Mg++, Cu++, Mn++ inhibit the enzyme activity. Also reducing agents and inhibitors were tested. Reducing agent such as Dithiothreitol (DTT) inhibit the enzyme activity whereas sodium sulphide, SDS increases the enzyme activity. 1,10phenanthroline and EDTA were increases the enzyme activity.


Keywords: Actinomycetes, Streptomyces, Keratinase , Feather meal.
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How to cite this article:

Jadhav, R.S., D.D. Karad and Kulakrni, S.W. 2016. Isolation, Identification and Characterization of Keratinolytic Streptomyces coelicoflavus.Int.J.Curr.Microbiol.App.Sci. 5(7): 153-163. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.015