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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 293-298
DOI: https://doi.org/10.20546/ijcmas.2018.702.038


Mupirocin Resistance in Staphylococcus aureus isolated from the Anterior Nares of Health Care Workers, in a Tertiary Care Hospital
Ragini Ananth Kashid1* and Kausalya Raghuraman2
1Department of Microbiology, BGS Global Institute of Medical Sciences, No.67, BGS Health and Education City, Uttarahalli Main Road, Kengeri Bangalore – 560060, India
2Department of Microbiology, Raja Rajeswari Medical College and Hospital, Kambipura, Mysore Road, Bangalore-560074, India
*Corresponding author
Abstract:

Nasal carriage of methicillin resistant Staphylococcus aureus (MRSA) is a key factor in the epidemiology and causation of infection. Health care workers, who are nasal carriers of MRSA, act as reservoirs of infection. This is an important risk factor in the development of nosocomial infections. Mupirocin is used to decolonise MRSA carriers. But with the widespread use of mupirocin, resistance to mupirocin has set in. This poses a challenge to the use of mupirocin against MRSA. This study was carried out to know the occurrence of high and low level mupirocin resistance in Staphylococcus aureus. Staphylococcus aureus isolated from the anterior nares of health care workers (HCWs) were identified using standard protocol. Methicillin resistance was identified using cefoxitin disc (30µg). High level and low level mupirocin resistance was identified using 5µg and 200µg mupirocin discs. The tests were done by Kirby Bauer disc diffusion method as per CLSI guidelines. SPSS Version 20 software was used to give percentage analysis of the data. Chi-square test was used for statistical analysis (p value <= 0.05 is taken as statistically significant). Of the 300 nasal swabs taken from HCWs, 28 Staphylococcus aureus were isolated, of which 24 isolates were MSSA and 4 isolates were MRSA. One MSSA (1/24, 4.1%) isolate showed high level mupirocin resistance. Only 1 MRSA isolate (1/4, 25%) had high level resistance to mupirocin. No low level resistance to mupirocin was detected in the MSSA and MRSA isolates. Presence of mupirocin resistance in Staphylococcus aureus isolates is a cause of grave concern. The hospital infection control activities and surveillance have to be stepped up, to identify and control mupirocin resistance.


Keywords: Anterior nares, Health care workers, High level mupirocin resistance, Low level mupirocin resistance, Methicillin resistant, Staphylococcus aureus
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How to cite this article:

Ragini Ananth Kashid and Kausalya Raghuraman. 2018. Mupirocin Resistance in Staphylococcus aureus Isolated from the Anterior Nares of Health Care Workers, in a Tertiary Care Hospital.Int.J.Curr.Microbiol.App.Sci. 7(2): 293-298. doi: https://doi.org/10.20546/ijcmas.2018.702.038