Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 177-192
DOI: https://doi.org/10.20546/ijcmas.2018.702.023


Wheat Oxygen Evolving Enhancer Protein: Identification and Characterization of Mn-Binding Metalloprotein of Photosynthetic Pathway Involved in Regulating Photosytem II Integrity and Network of Antioxidant Enzymes under Heat Stress
Ansif Ali1, Suneha Goswami1*, Ranjeet R. Kumar1, Khushboo Singh1, Jyoti P. Singh1, Ashok Kumar1, Arti Kumari1, Akshay Sakhrey2, Gyanendra K. Rai3 and Shelly Praveen1*
1Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, India
2Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, India
3School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, India
*Corresponding author
Abstract:

PsbO is a ubiquitous extrinsic protein of photosystem II required for providing proper environment for water splitting activity of photosystem II. Exposure of wheat to heat stress initially reduces the rate of photosynthesis by disintegrating the photosystem II, primarily by disruption of the oxygen evolving complex (OEC). Here, we identified and cloned putative PsbOgene of 1 kb from wheat cv. HD2329 using de novo assembly. Expression analysis showed higher expression of PsbO transcript in HD2985, as compared to HD2329 during pollination and grain-filling stages. We observed significant variations in the expression of PsbO in response to differential HS. A positive correlation was established between the expression of PsbO and activity of antioxidant enzymes like superoxide dismutase, catalase and guaiacol peroxidase in response to heat stress in contrasting wheat cvs. at different stages of growth. Photosynthetic rate was observed slightly higher in thermotolerant cultivar at different stages, as compared to susceptible. There is, however, need to discover many more isoforms of PsbO from wheat in order to characterize their role in the modulation of carbon assimilatory pathway under heat stress. Maintaining the integrity of PSII under HS using PsbO will pave the way for the development of ‘climate-smart’ wheat.


Keywords: Catalase, Heat stress, Peroxidase, photosynthetic rate, IRGA, PsbO, qRT-PCR, Superoxide dismutase, Total antioxidant activity
Download this article as Download

How to cite this article:

Ansif Ali, Suneha Goswami, Ranjeet R. Kumar, Khushboo Singh, Jyoti P. Singh, Ashok Kumar, Arti Kumari, Akshay Sakhrey, Gyanendra K. Rai and Shelly Praveen. 2018. Wheat Oxygen Evolving Enhancer Protein: Identification and Characterization of Mn-Binding Metalloprotein of Photosynthetic Pathway Involved in Regulating Photosytem II Integrity and Network of Antioxidant Enzymes under Heat Stress.Int.J.Curr.Microbiol.App.Sci. 7(2): 177-192. doi: https://doi.org/10.20546/ijcmas.2018.702.023