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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 3808-3815
DOI: https://doi.org/10.20546/ijcmas.2017.609.470


Growth Kinetics of V. parahaemolyticus in Post-Harvest Shellstock Clams (Meretrix meritrx) Spiked with Pathogenic Strains of V. parahaemolyticus
V. Alamelu1*, B. K. Krishna2, Dechamma Sony2, M. N. Venugopal2 and S. Malathi2
1Fisheries College & Research Institute, TNFU, Thoothukudi – 628 008, Tamil Nadu, India
2Department of Fisheries Microbiology, College of Fisheries, KVAFSU, Mangalore–575 002, Karnataka, India
*Corresponding author
Abstract:

Pathogenic strains of V. parahaemolyticus exhibits growth variability at different storage temperatures and it plays an vital role in seafood safety risk assessment. The present study investigated the effects of storage temperatures on growth kinetics of total V. parahaemolyticus in artificially spiked post-harvest shell stock clams (Meretrix meretrix). Clams are consumed raw or undercooked. Vibrios accumulates in clams and that may exceed levels that cause human illness when post harvest temperatures are not properly controlled. Growth kinetics were produced by injecting clams with three different strains of V. parahaemolyticus and measuring viability rates at temperatures (10, 20, 30 and 37°C) at selected time intervals. At selected time intervals, separated samples of five clams, each were homogenated and analyzed for total V. parahaemolyticus count. The results revealed that the growth variability among strains increased as the growth conditions became more stressful in terms of temperature (20°C and 10°C). This study confirms that the storage temperatures as well as strain variability of V. parahaemolyticus have impacts on growth kinetics of total V. parahaemolyticus and which can be helpful for incorporating strain variability in microbial risk assessment.


Keywords: V. parahaemolyticus, storage temperature, growth kinetics, environmental factor, strain variability
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How to cite this article:

Alamelu V., B. K. Krishna, Dechamma Sony, M. N. Venugopal and Malathi S. 2017. Growth Kinetics of V. parahaemolyticus in Post-Harvest Shellstock Clams (Meretrix meritrx) Spiked with Pathogenic Strains of V. parahaemolyticus.Int.J.Curr.Microbiol.App.Sci. 6(9): 3808-3815. doi: https://doi.org/10.20546/ijcmas.2017.609.470