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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 3583-3591
DOI: https://doi.org/10.20546/ijcmas.2018.701.420


In vitro Root Development System in Two Popular Cultivars of Banana
Mulugeta Gobena, Sunil T. Hajare* and Girmaye Benti
Department of Biology, College of Natural and Computational Sciences,Dilla University, Dilla 419, Ethiopia
*Corresponding author
Abstract:

Banana is one of the most important and remunerative cash crops grown round the year in Ethiopia. The energy and nutritional status of banana are much higher than other common tropical and subtropical fruits. The objective of this study was to establish in vitro root development protocol in Poyo and Grand naine varieties from sucker. For root induction suckers of Poyo and Grand naine were cultured on MS basal medium that contained 30 g/l sucrose, 6 g/l agar and vitamins. Rooting of the shoots was done on full strength MS basal medium supplemented with different concentrations of IBA (0.5, 1.0, 1.5, 2.0 mg/l) and NAA in sole and in combination (0.5 mg/l IBA+ 0.5 mg/l NAA and 1.0 mg/l IBA + 1.0 mg/l NAA). In all cases, MS basal medium without PGRs was used as control. The rooted plantlets were then acclimatized under greenhouse conditions by transplanting the plantlets on moist red soil, sand soil and compost or their mixture in 2:1:1 ratio. Results showed that root formation responses were significantly (P<0.05) higher under all levels and combinations of hormones when compared with control treatments. For Poyo variety, 0.5mg/l IBA had higher response for root numbers and root length than at other treatments. Number of days to root emergence was found to be shorter at 20.mg/l NAA of hormonal level than other treatments. In the case of Grand naine variety, the concentration of 1.5mg/l IBA alone treatment had positive effects on multiple root formation and MS + 0.5 mg/l IBA for highest length of roots. The number of days for root emergence was found to be shorter at combination of 1.0 + 1.0mg/l IBA and NAA in grand naine. Compared to the control, both IBA and NAA alone or in combination showed significant positive effect on root formation of shoots of both varieties. The acclimatization experiment showed that plantlets of both varieties survived better on the sterilized mixture of moist red soil, sand and compost soil in a ratio of 2:1:1. Comparison of the performance of both varieties showed that Poyo showed superior performance compared to Grand naine variety with respect to all measured agronomic traits except field survival rate which found better in grand naine, suggesting that varietal difference for the difference in the response to the PGRs.


Keywords: In vitro rooting, PGR, Musa spp, Poyo, Grand naine, IBA, NAA
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How to cite this article:

Mulugeta Gobena, Sunil T. Hajare and Girmaye Benti. 2018. In vitro Root Development System in Two Popular Cultivars of Banana.Int.J.Curr.Microbiol.App.Sci. 7(1): 3583-3591. doi: https://doi.org/10.20546/ijcmas.2018.701.420