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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 3339-3353
DOI: https://doi.org/10.20546/ijcmas.2018.701.396


Expression of Bt Gene (cry1Ac) on Different Plant Parts at Different Stages in Bt Cotton Genotypes
 
B.H. Nagappa* and B.M. Khadi
Agricultural Research Station (UAS, Dharwad), Hanumanamatti, Ranebennur Taluk,Haveri District, India
*Corresponding author
Abstract:

Cotton yield losses due to insect pests are estimated to be around 10 to 14 per cent every year. These insect protected cotton varieties contain a naturally occurring substance, Bacillus thuringiensis (Bt) protein which has been used as an ingredient in safe and effective biological sprays for more than 50 years. Of the total available Bt cotton hybrids in 2006, four different events containing Cry1Ac gene (MON 531 event) by Mahyco sourced from Monsanto, stacked Cry X (Cry1Ac and Cry2Ab) gene event (MON 15985) developed by Mahyco sourced from Monsanto, Cry1Ac gene (Event 1) by JK-seeds sourced from IIT Kharagpur and fusion gene Cry1Ab and Cry1Ac (GF Mervent) by Nath seeds sourced from China have received commercial approval for sale in Northern, Central and Southern cotton growing zones. The expression of Bt gene varies with different parts of plant.  The Cry 1 Ac gene expression highest in leaf (4.31 mg/g at 45 DAS) followed by young boll (2.02 mg/g at 105 DAS), flower (> 1.9 mg/g 105 DAS), seeds (1.9 mg/g 120 DAS and 1.49 mg/g at 160 DAS and least in rind (0.63 mg/g 105 DAS).


Keywords: Bt Gene (cry1Ac), Bt Cotton Genotypes, Gene expression
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How to cite this article:

Nagappa, B.H. and Khadi, B.M. 2018. Expression of Bt Gene (cry1Ac) on Different Plant Parts at Different Stages in Bt Cotton Genotypes.Int.J.Curr.Microbiol.App.Sci. 7(1): 3339-3353. doi: https://doi.org/10.20546/ijcmas.2018.701.396