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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 3150-3155
DOI: https://doi.org/10.20546/ijcmas.2018.701.373


Diagnosis of Human Toxoplasmosis Using Rapid Chromatographic Immunoassay and Enzyme-Linked Immuno-Sorbent Assay (ELISA) Compared to Molecular Technique (PCR) as Gold Standard Technique
Kareem Abdal Razaq Mouhamed1, Abdel-Kareem A. AL-Kazzaz2 and Atif S.M. Idrees3
1College of Graduate Studies, Al-Neelain University Sudan
2Department of Biotechnology, College of Science, University of Baghdad, Iraq
3Department of Biology and Biotechnology, Faculty of Science and Technology,Al-Neelain University, Sudan
*Corresponding author
Abstract:

Nowadays, there are many different procedures for the laboratory diagnosis of Toxoplasma infection in pregnant women, congenitally-infected fetuses and newborns, and they are mainly performed by serological testing, and PCR (for confirmatory purpose), beside many commercial diagnostic tests, which use Toxoplasma lysate antigen. These procedures differ from each other in many aspects; cost, time-consumption, and accuracy of the test, which should meet patient’s needs. In this current study, serum samples were collected from one-hundred females suspected with toxoplasmosis and diagnosed by three different procedures for the T. gondii infection, rapid chromatographic (IgM or IgG immunoblot), ELISA (IgM or IgG) test and molecular technique. Molecular diagnoses were performed in peripheral blood by PCR using the T. gondiiB1 gene as marker. The results were described as frequency and percentage of positivity; also, specificity and sensitivity were assessed. Of these 100 blood samples analyzed, 92% (IgG), and 55% (IgM) were positive when using PCR; the rapid chromatographic method for both IgM and IgG, has shown (83%) samples to be positive for T. gondii, while in ELISA test, 28% (IgM) and 72% (IgG) found to be positive. Sensitivity and specificity of ELISA (89% and 90% (IgM); 89% and 91% (IgG), respectively) have found to be relatively higher than immunoblot (88%and81% (IgM); 85% and 81% (IgG), respectively). While PCR technique has shown 100% and 98.8% (IgM); 99.3% and 96.1% (IgG), sensitivity and specificity, respectively. Since the higher sensitivity and specificity of ELISA, we concluded that ELISA, compared to the rapid chromatographic test, is more suitable for the detection of anti-T. gondii IgG and IgM antibodies in both acute and chronic infection, especially, the rapid chromatographic commercial kits can yield many false-positive results which in turn has many undesired consequences.


Keywords: Toxoplasmosis, T. gondii, Diagnosis, Immunoblottest, ELISA, PCR.
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How to cite this article:

Kareem Abdal Razaq Mouhamed, Abdel-Kareem A. AL-Kazzaz and Atif S.M. Idrees. 2018. Diagnosis of Human Toxoplasmosis Using Rapid Chromatographic Immunoassay and Enzyme-Linked Immuno-Sorbent Assay (ELISA) Compared to Molecular Technique (PCR) as Gold Standard Technique.Int.J.Curr.Microbiol.App.Sci. 7(1): 3150-3155. doi: https://doi.org/10.20546/ijcmas.2018.701.373