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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 61-70
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.005


Studies on Cytotoxic Effects of Ochratoxin A Extracts on Human Cell Lines (Hfl-1 and Hep-2)
Jamal I. Elzwai1, Mohamed A. Ahmed1, Kofi A. Aidoo2, Allan A. Candlish2 and Alyaa M. Homoud3*
1Food Technology Department, Faculty of Engineering and Technology, Sebha University
2Biological and Biomedical Dept. School of life Sciences, Glaasgow Caledonian University, Glasgow, UK
3Home Economic Department (Nutrition), Faculty of science and art, Al Baha University, KSA
*Corresponding author
Abstract:

Ochratoxin A (OTA), the mycotoxin produced by species of Aspergilli, specifically Aspergillus carbonarius  and Aspergillus ochraceus, and Penicillium verrucosum have harmful effects on humans, animals, and crops that result in illnesses and economic losses. Therefore, this study has been undertaken the cytotoxic effects of ochratoxin A of the four moulds extracts which has been used in this study in two human cell lines(Hfl-1 and Hep-2). Control experiment (uncontaminated solid substrate extract) showed negligible cytotoxic effects on both cell lines after 72 hr exposure. All mould (OTA) extracts examined showed a cytotoxic effect on both cell lines, although it is possible that other compounds in the extracts may have contributed to the cytotoxic effects. Effect of the toxin extracts on the cell lines increased with incubation time. Aspergillus ochraceus J002 and A. ochraceus strain CBS 588.68 showed higher cytotoxic effects on Hfl-1 than A. carbonarius J001 and P. verrucosum strain IMI 285522 whereas on Hep-2, A. carbonarius J001 and A. ochraceus strain CBS 588.68 showed higher cytotoxic effects than A. ochraceus J002 and P. verrucosum IMI 285522. IC50 ranged between 1.37 to 38.87 using Hfl-1 cells and 1.94 to 50.0 using Hep-2 cells. IC50 values by Aspergillus ochraceus strain CBS 588.68 was not determined after 72hr, this may have been due to higher concentration of OTA in the extract than suspected.


Keywords: Ochratoxin A Aspergillus carbonarius, Aspergillus ochraceus, Penicillium verrucosum.
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How to cite this article:

Jamal I. Elzwai, Mohamed A. Ahmed, Kofi A. Aidoo, Allan A. Candlish and Alyaa M. Homoud. 2016. Studies on Cytotoxic Effects of Ochratoxin A Extracts on Human Cell Lines (Hfl-1 And Hep-2).Int.J.Curr.Microbiol.App.Sci. 5(7): 61-70. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.005