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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 3060-3071
DOI: https://doi.org/10.20546/ijcmas.2018.701.363


A Non-Pathogenic Environmental Isolate of Pseudomonas aeruginosa MCCB 123 with Biotechnological Potential
Divya Jose, A. Mohandas and I.S. Bright Singh*
National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Lakeside Campus, Fine Arts Avenue, Cochin – 682016, India
*Corresponding author
Abstract:

Pseudomonas aeruginosa MCCB 123 is a potent producer of enzymes such as β-1,3 glucanase, LasA and LasB proteases, which have been identified to have greater significance in the biotechnology sector. However, pathogenicity assessment of bacteria is essential for its industrial survival. Pathogenicity was assessed by a panel of virulence assays such as the presence of type III secretion toxin genes, motility assays, biofilm formation, adhesion and invasion assays on Hep-2 and HeLa monolayers and antibiogram profiling. Analysis of the major cytotoxic exoU gene revealed the absence of the gene, thus confirms the non-cytotoxic phenotype of the bacterium. P. aeruginosa exhibited three types of motilities viz., swimming, swarming and twitching motilities and proved to be a moderate biofilm producer with a 3.10±0.52-fold increase in the optical density at Abs570 when compared to control. Antibiogram suggested the possibility of antibiotic treatment as an effective method for eradication of its biofilm. The non-pathogenic nature of the bacterium suggests industrially viability of this organism for the production of biotechnological relevant enzymes.


Keywords: Pseudomonas aeruginosa, β-1,3 glucanase, LasA protease, LasB protease, biotechnology
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How to cite this article:

Divya Jose, A. Mohandas and Bright Singh, I.S. 2018. A Non-Pathogenic Environmental Isolate of Pseudomonas aeruginosa MCCB 123 with Biotechnological Potential.Int.J.Curr.Microbiol.App.Sci. 7(1): 3060-3071. doi: https://doi.org/10.20546/ijcmas.2018.701.363