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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 3023-3030
DOI: https://doi.org/10.20546/ijcmas.2018.701.359


Comparison of Various Phenotypic Methods in Detection of Carbapenemases and Metallo-Beta-Lactamases in Carbapenem Resistant Clinical Isolates of Acinetobacter Species at a Tertiary Care Centre in North India
Ankur Goyal1*, Neha K Mani1, Renu Chahar1, Ankita Soni1 and Sapna Goyal1
Department of Microbiology, Sarojini Naidu Medical College, Agra, Uttar Pradesh, India
*Corresponding author
Abstract:

Carbapenem resistance has become a worldwide issue and a major reason of concern for the treating physicians. Plasmid mediated metallo-beta-lactamases (MBLs) as well as oxacillinases (OXA) are responsible for carbapenem resistance. Various phenotypic as well as genotypic screening methods are used for detecting carbapenemases and MBLs. The strains were first and foremost screened for carbapenem resistance by Kirby-Bauer Disc Diffusion method. The resistant strains were detected for the production of carbapenemases by Modified Hodge Test (MHT) followed by MBL detection using Disc Potentiation Test (DPT) and EDTA Disc Synergy (EDS) test simultaneously. Our study emphasizes on the detection of carbapenem resistant Acinetobacter sps. emerging due to MBL production, and also for understanding the impact of MBL production in carbapenem resistant strains. Among the 100 clinical isolates of carbapenem resistant Acinetobacter sps. subjected to the phenotypic detection of carbapenemases and metallo-beta-lactamases, carbapenemases production was found to be 86 per cent by MHT, MBL production as 43 per cent and 86 per cent by DPT and EDS respectively. Thus, according to our study, EDS was considered to be more sensitive and reliable phenotypic test for MBL detection in our isolates.


Keywords: Carbapenem resistance, Metallo-beta-lactamases (MBLs), Enzyme carbapenemases, Modified Hodge Test (MHT), Disc Potentiation Test (DPT), EDTA Disc Synergy (EDS)
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How to cite this article:

Ankur Goyal, Neha K. Mani, Renu Chahar, Ankita Soni and Sapna Goyal. 2018. Comparison of Various Phenotypic Methods in Detection of Carbapenemases and Metallo-Beta-Lactamases in Carbapenem Resistant Clinical Isolates of Acinetobacter Species at a Tertiary Care Centre in North India.Int.J.Curr.Microbiol.App.Sci. 7(1): 3023-3030. doi: https://doi.org/10.20546/ijcmas.2018.701.359