National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Carbapenem resistance has become a worldwide issue and a major reason of concern for the treating physicians. Plasmid mediated metallo-beta-lactamases (MBLs) as well as oxacillinases (OXA) are responsible for carbapenem resistance. Various phenotypic as well as genotypic screening methods are used for detecting carbapenemases and MBLs. The strains were first and foremost screened for carbapenem resistance by Kirby-Bauer Disc Diffusion method. The resistant strains were detected for the production of carbapenemases by Modified Hodge Test (MHT) followed by MBL detection using Disc Potentiation Test (DPT) and EDTA Disc Synergy (EDS) test simultaneously. Our study emphasizes on the detection of carbapenem resistant Acinetobacter sps. emerging due to MBL production, and also for understanding the impact of MBL production in carbapenem resistant strains. Among the 100 clinical isolates of carbapenem resistant Acinetobacter sps. subjected to the phenotypic detection of carbapenemases and metallo-beta-lactamases, carbapenemases production was found to be 86 per cent by MHT, MBL production as 43 per cent and 86 per cent by DPT and EDS respectively. Thus, according to our study, EDS was considered to be more sensitive and reliable phenotypic test for MBL detection in our isolates.
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