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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 2876-2881
DOI: https://doi.org/10.20546/ijcmas.2018.701.342


In-House Standardization of Carba NP Test for Carbapenemase Detection in Gram Negative Bacteria
Mangayarkarasi*, Stephen Pradeep Moses, S.R. Swarna,Kalaiselvi and S. Shaweez Fathima
Department of Microbiology, SRM Medical College Hospital and Research Centre, Kattankulathur-603203, Chennai, India
*Corresponding author
Abstract:

This study was done to evaluate the performance of Carba NP test for the detection of carbapenemase producing Gram negative bacilli. In total, 330 Gram negative bacilli isolates were subjected to Antibiotic Susceptibility Testing, Epsilometer test, Modified Hodge test with meropenem to determine the presence of resistance among carbapenem group of drugs. 44/ 330(13.3%) showed resistance pattern. The 44 resistant isolates were then subjected to the Carba NP test and incubated at 37°C and results were read after 2 hours. According to the principle of Carba NP test there was a color change in all 44 isolates indicating a sensitivity of 100%. Overall, the Carba NP test is a useful tool for rapid detection of carbapenemase activity.


Keywords: Carba NP test, Modified Hodge test, Epsilometer test, Carbapenem resistance, Antibiotic susceptibility testing
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How to cite this article:

Mangayarkarasi, V., Stephen Pradeep Moses, S.R. Swarna, K. Kalaiselvi and Shaweez Fathima, S. 2018. In-House Standardization of Carba NP Test for Carbapenemase Detection in Gram Negative Bacteria.Int.J.Curr.Microbiol.App.Sci. 7(1): 2876-2881. doi: https://doi.org/10.20546/ijcmas.2018.701.342