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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 2813-2823
DOI: https://doi.org/10.20546/ijcmas.2018.701.336


Optimization of Cultural Conditions for High Production of Antifungal Activity by Fluorescent Pseudomonas sp. against Dematophora necatrix and Phytophthora cactorum
Pankaj Prakash Verma1*, Parul Sharma2 and Mohinder Kaur2
1Department of Microbiology, Kurukshetra University, Kurukshetra, Haryana, India
2Department of Basic Science (Microbiology Section), Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan-173230, Himachal Pradesh, India
*Corresponding author
Abstract:

The antagonistic activities of fluorescent Pseudomonas sp. isolated from rhizospheric soil of apple orchard of Himachal Pradesh were studied against two fungal pathogens viz., Dematophora necatrix and Phytophthora cactorum which were previously isolated from apple rhizosphere. The optimum conditions for growth and maximum production of antifungal activity by two selected fluorescent Pseudomonas sp. viz., An-2-nali and Pn-2-kho were detected. The influence of culture media, incubation time, incubation temperature and pH on growth and production of antifungal activity was investigated. From the results it is concluded that out of five media i.e. Potato dextrose agar (PDA), Kings’ B, Pigment production media (PPM), Yeast malt (YM) and 1/5 M 523, the maximum production of antifungal activity in terms of percent inhibition was observed in Yeast malt media after 96 hr of incubation. An-2-nali showed 37.93 %I against Dematophora necatrix and 38.46 %I against Phytophthora cactorum whereas isolate Pn-2-kho showed 43.10 %I against Dematophora necatrix and 36.53 %I against Phytophthora cactorum. The optimum conditions for production of antifungal activity by both the fluorescent Pseudomonas isolates were observed to be at temperature 28ËšC, pH 7 and an incubation time of 96 hr.


Keywords: Cultural conditions, Phytopathogenic fungi, Pseudomonas sp., Secondary metabolites
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How to cite this article:

Pankaj Prakash Verma, Parul Sharma and Mohinder Kaur. 2018. Optimization of Cultural Conditions for High Production of Antifungal Activity by Fluorescent Pseudomonas sp. against Dematophora necatrix and Phytophthora cactorum.Int.J.Curr.Microbiol.App.Sci. 7(1): 2813-2823. doi: https://doi.org/10.20546/ijcmas.2018.701.336