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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 2501-2508
DOI: https://doi.org/10.20546/ijcmas.2018.701.300


Isolation and Identification of Phenol Degrading Halophilic Bacteria from Lonar Lake
B.A. Mehere, Priyanka A. Gupta* and Manashree D. Dongre
Department of Biochemistry and Biotechnology, Dr. Ambedkar College, Affiliated to RTM Nagpur University, Nagpur, India
*Corresponding author
Abstract:

Phenol and its components are extremely toxic and can easily be isolated from different industrial sewage such as oil refinery, petrochemical industry and mines, especially chemical factories causing pollution in water bodies. Rivers and oceans are contaminated on a large scale with these toxic compounds. In the past, physicochemical method was used for the elimination of phenol and its compounds, but today, bioremediation is preferable. The aim of this study is to isolate promising halophilic bacteria from Lonar Lake showing phenol degradation in wide pH range (neutral as well as alkaline). Different isolates from Lonar Lake were tested for growth in presence of phenol as sole C source. The isolate growing in high phenol concentration was identified on basis of biochemical identification methods and 16s sequencing. Finally, the ability of bacteria to degrade different concentration of phenol was measured using culturing bacteria in different concentration of phenol from 0.3 to 1.0 g/l. and turbidity was measured after 24-48 days. The phenol degradation was estimated using Folin Lowry method after 48 hrs of inoculation. The isolated bacteria was identified as Halomonas campisalis.


Keywords: Lonar Lake, Bioremediation, Phenol degradation and Halomonas campisalis
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How to cite this article:

Mehere, B.A., Priyanka A. Gupta and Manashree D. Dongre. 2018. Isolation and Identification of Phenol Degrading Halophilic Bacteria from Lonar Lake.Int.J.Curr.Microbiol.App.Sci. 7(1): 2501-2508. doi: https://doi.org/10.20546/ijcmas.2018.701.300