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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 2397-2404
DOI: https://doi.org/10.20546/ijcmas.2018.701.288


Comparison of Rate of Isolation and Characterisation of Mycobacteria by AFB Smear, LJ Medium and MGIT
Pratibha Bhat1* and Rekha Boloor2
1Department of Microbiology, K S Hegde Medical Academy, Mangaluru, India
2Department of Microbiology, Father Muller Medical College, Mangaluru, India
*Corresponding author
Abstract:

Bacteriological confirmation plays a key role in the diagnosis of tuberculosis. Many systems have been developed in the recent years apart from traditional microscopy and culture on Lowenstein Jensen (LJ) medium. The objective of this study is to compare the rate of detection of Mycobacteria by acid fast staining, LJ medium and Mycobacteria Growth Indicator Tube (MGIT). Hundred patients with clinical suspicion of pulmonary tuberculosis attending a tertiary care hospital over a period of one and a half years were enrolled in the study. Sputum samples were collected and acid fast smears were prepared. The specimens were decontaminated and cultured on LJ medium and MGIT. Growth obtained was recorded. Out of 100 suspected cases, acid fast bacilli were seen in 21 sputum samples by Ziehl-Neelsen (ZN) staining. In LJ medium culture 21 samples yielded growth and MGIT was positive in 23 samples. The sensitivity and specificity of MGIT in comparison with LJ medium culture were 100% and 97.5% respectively. MGIT culture showed faster rates of isolation compared to LJ medium with a mean detection time of 10.91days. Among the tuberculosis positive cases, 18 (78.2%) were males and 5 (21.7%) were females, with peak incidence (43.47%) in age group of 31-45years. Compared to conventional methods, MGIT showed a good sensitivity and specificity and faster rates of isolation. It can be used in the diagnosis of tuberculosis as a rapid and reliable technique.


Keywords: Tuberculosis, MGIT, Diagnosis
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How to cite this article:

Pratibha Bhat, U. and Rekha Boloor. 2018. Comparison of Rate of Isolation and Characterisation of Mycobacteria by AFB Smear, LJ Medium and MGIT.Int.J.Curr.Microbiol.App.Sci. 7(1): 2397-2404. doi: https://doi.org/10.20546/ijcmas.2018.701.288