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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 2336-2347
DOI: https://doi.org/10.20546/ijcmas.2018.701.282


Morphological and Molecular Identification of Arbuscular Mycorrhizal (AM) Fungi
Bindu R. Goswami, Manoj V. Parakhia, B.A. Golakiya and Charmy R. Kothari*
Department of Biotechnology, Junagadh Agriculture University, Junagadh-362001, India
*Corresponding author
Abstract:

Mycorrhiza is important fungi for agriculture. To study of that plant’s root and soil samples were collected from the different regions of junagadh and near to junagadh district. AMF spores were isolated by Wet-sieving-decanting method and Adhesion-Flotation Technique. Morphological studies followed by tryphan blue staining revealed different stages with different components of AM fungi in compound microscope. Different size of spores 10 um, 20 um, 53 um and 105 um were observed in Scanning Electron Microscope (SEM). DNA of AMF colonized root were extracted by CTAB method. Using universal primers (A) AML1 and AML2 (B) NS 1 and NS 4 have been used for the identification of AM fungi they generated a single clear band around 800 bp and 1100 bp. For identification of specific mycorrhizal species Glomus, Aculospora or Gigaspora specific primers ITS1F, ITS4R, GLOM1310F, GLOM5.8R, GIGA5.8R, ARCH1311F, ACAU1660F and LETC1670F used.


Keywords: Arbuscular Mycorrhizal (AM), Molecular
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How to cite this article:

Bindu R. Goswami, Manoj V. Parakhia, B.A. Golakiya and Charmy R. Kothari. 2018. Morphological and Molecular Identification of Arbuscular Mycorrhizal (AM) Fungi.Int.J.Curr.Microbiol.App.Sci. 7(1): 2336-2347. doi: https://doi.org/10.20546/ijcmas.2018.701.282