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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 2227-2232
DOI: https://doi.org/10.20546/ijcmas.2018.701.268


Mass Production of Entomopathogenic Fungi (Metarhizium anisopliae) using Different Grains as a Substrate
S.V. Agale1*, S. Gopalakrishnan2, K.G. Ambhure1,Hemkant Chandravanshi1, Rajeev Gupta1 and S.P. Wani2
 
1Department of Entomology, Indira Gandhi Krishi Vishwavidyalaya, Raipur, 492012, Chhattisgarh, India
2International Crops Research Institute for the Semi-Arid Tropics (ICRISAT),Patancheru 502324, Hyderabad, Telangana, India
*Corresponding author
Abstract:

Investigations were carried out towards the “Mass production of entomopathogenic fungi (Metarhizium anisopliae) using different grains as a substrate” at International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad during 2016-17. The accomplishment of microbial control of insect pests, the successful mass production of the microbial agents in the laboratory significant availability of the pathogen is a primary requirement in the biocontrol programme. Metarhizium anisopliae is an entomopathogenic fungus which is used against a number of insect pests’ management and very successful biopesticides in integrated pest management practices to reduces the cost of production and minimize the environment and public health hazard. To develop a proficient method for the deployment of this fungus as a bio control agent, various grains and liquid media such as Potato Dextrose Broth and Sabouraud’s Dextrose Broth were screened. Significantly highest conidial count (67.6 × 103 spores/ml) was observed on green gram followed by sorghum in 103 dilutions, the assessment fungus whereas SDB produced significantly higher spore production of the fungi. Highest conidial count (63.7 × 103 spores/ml) was observed on SDB media followed by PDB in 103.


Keywords: Metarhizium anisopliae, Grain substrates, Mass production
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How to cite this article:

Agale, S.V., S. Gopalakrishnan, K.G. Ambhure, Hemkant Chandravanshi, Rajeev Gupta and Wani, S.P. 2018. Mass Production of Entomopathogenic Fungi (Metarhizium anisopliae) using Different Grains as a Substrate.Int.J.Curr.Microbiol.App.Sci. 7(1): 2227-2232. doi: https://doi.org/10.20546/ijcmas.2018.701.268