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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 1767-1775
DOI: https://doi.org/10.20546/ijcmas.2018.701.214


In Vitro Cytotoxic Activity of Chelidonium majus extract using Different Types of Cell Lines
Yasir B. Fadhil*, Khulood W. Alsammarraie, Nedhal Abdul Mohaimen and Zainab Yasin Mohammed
Department of Biotechnology, College of Science, Al-Nahrain University, Baghdad, Iraq
*Corresponding author
Abstract:

Cancer is becoming a health problem in all over the world, searching for anticancer therapy from plants natural products has been one of the sustainers of cancer chemotherapy approximately for the past half a century. Chelidonium majus (Poppy Family) is a plant which is distributed in nature and has been used as a traditional medicine. The plant extract showed high content of alkaloids which revealed cytotoxic effect in a cell type and dose dependent way. Cytotoxic analysis of alkaloids extract from Chelidonium majus using MTT assay on different cell lines. 100 grams of the plant material powdered and extracted with methanol using Soxhlet installation, the extract was subjected farther more for acid-base extraction and purification with chloroform. Cytotoxic effect of total alkaloid was estimated using MTT-assay manufacturer’s instructions. The total alkaloid extract of C. majus exhibited remarkably significant cytotoxic effect on (Hep-G2, MCF-7 and A-549) cell lines in a dose dependent and cell type dependent manner. The extract illustrated considerable cytotoxic activity against various types of cancer cell lines in vitro. The antitumor activity was extremely efficient in a dose dependent manner it displayed decreased viability of tumor cells.


Keywords: Chelidonium majus, Cell culture, MTT assay
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How to cite this article:

Yasir B. Fadhil, Khulood W. Alsammarraie, Nedhal Abdul Mohaimen and Zainab Yasin Mohammed. 2018. In vitro Cytotoxic Activity of Chelidonium majus Extract Using Different Types of Cell Lines.Int.J.Curr.Microbiol.App.Sci. 7(1): 1767-1775. doi: https://doi.org/10.20546/ijcmas.2018.701.214