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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 1737-1743
DOI: https://doi.org/10.20546/ijcmas.2018.701.210


Antibiotic Resistance in Bacterial Isolates from Commercial Probiotics Used in Aquaculture
A. Uma* and G. Rebecca
State Referral Laboratory for Aquatic Animal Health, Tamil Nadu Fisheries University-Madhavaram Campus, Madhavaram Milk Colony, Chennai-600051, Tamil Nadu, India
*Corresponding author
Abstract:

Aquaculture involves farming of commercially important species of finfish and shellfish. Intensification of aquaculture practices has led to many diseases of commercial importance. Probiotics are beneficial microorganisms used in aquaculture to improve the health and disease resistance of the cultured fish. It is considered as a safe alternative to antibiotics which confer resistance in the microbes when used indiscriminately. In the present study, bacterial isolates (PB-1, PB-2, PB-3, PB-4 and PB-5) from the selected commercial probiotic products used in aquaculture were assessed for their bacterial pathogen inhibition efficiency, antibiotic sensitivity and the presence of antibiotic resistance genes viz., tetK, tetL, tetM and tetO. The probiotic isolates exhibited varying levels of inhibition against the bacterial pathogens viz., Aeromonas hydrophila, Streptococcus sp., Flavobacterium columnare, Edwarsiella tarda and Vibrio alginolyticus. F.columnare was the most inhibited by all the five probiotic bacterial isolates. The antimicrobial sensitivity test showed that the isolates, PB-2, PB-3, PB-4 and PB-5 were resistant to oxytetracycline except the isolate, PB-1. The antibiotic resistant genes, tetK and tetL were found to be present in all the probiotic bacterial isolates, whereas tetM and tetO were absent in all the isolates. The results of the study showed that although probiotics exhibit inhibitory activity against the bacterial pathogens, antibiotic resistance to oxytetracycline and the presence of antibiotic resistant genes showed that they could also act as a possible source for the transfer and spread of antibiotic resistance among the microbes in the aquatic ecosystem.


Keywords: Probiotics, Aquaculture, Antibiotic resistance, Antibiotic resistant genes
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How to cite this article:

Uma, A. and Rebecca, G. 2018. Antibiotic Resistance in Bacterial Isolates from Commercial Probiotics Used in Aquaculture.Int.J.Curr.Microbiol.App.Sci. 7(1): 1737-1743. doi: https://doi.org/10.20546/ijcmas.2018.701.210