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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 1513-1522
DOI: https://doi.org/10.20546/ijcmas.2018.701.184


Validation of SSR Markers for Imparting Disease Resistance in Tomato (Solanum lycopersicum L.)
T.L. Dheemanth1*, P.A. Nazeem1, P.G. Sadhan Kumar2, Sally K. Mathewand M. Amaranatha Reddy4
1Centre for Plant Biotechnology and Molecular Biology, College of Horticulture, Kerala Agricultural University, Vellanikkara, Thrissur- 680 656, Kerala, India
2Department of Olericulture
3Department of Plant Pathology
4Department of Plant breeding, College of Horticulture, Kerala Agricultural University, Vellanikkara, Thrissur- 680 656, Kerala, India
*Corresponding author
Abstract:

The simple sequence repeat (SSR) or microsatellite marker is currently the most preferred molecular marker because of its reproducibility and codominant nature. The aim of this study was to validate SSR markers for bacterial wilt (BW) and tomato leaf curl virus disease (ToLCV) in tomato. DNA isolated from the parents Mukthi and IIHR-2195 was used to validate five SSR primers already reported for BW and ToLCV. One primer SSR20 which showed good polymorphism and reproducibility among parents were selected for further validation in F3 and F4 population. SSR20 was validated on resistant F4, their corresponding F3 parental lines, along with susceptible checks. The SSR20 segregated with the trait in the F3 and F4 resistant plants and was also found expressed in few susceptible checks. SSR20 identified in the study could be utilized for marker assisted selection with respect to bacterial wilt in tomato.


Keywords: SSR markers, Simple Sequence Repeat (SSR)
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How to cite this article:

Dheemanth, T.L., P.A. Nazeem, P.G. Sadhan Kumar, Sally K. Mathew and Amaranatha Reddy, M. 2018. Validation of SSR Markers for Imparting Disease Resistance in Tomato (Solanum lycopersicum L.).Int.J.Curr.Microbiol.App.Sci. 7(1): 1513-1522. doi: https://doi.org/10.20546/ijcmas.2018.701.184