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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 1457-1464
DOI: https://doi.org/10.20546/ijcmas.2018.701.177


Isolation of Thermophilic Bacteria and Optimizing the Medium Growth Conditions
Gustina Indriati and Ruth Rize Paas Megahati*
Biology Educational Department STKIP PGRI Sumbar, Padang, West Sumatera, Indonesia
*Corresponding author
Abstract:

The thermophile bacteria that came from hot spring, generally, it is thermostable, which it can produce enzyme. For example, there are protease, lipase, and amylase. Amylase produced by bacteria or bacteria amylase is an enzyme that can hydrolyze starch to sugar. The thermostable of amylase resist to high temperature and pH. It has been used on many industrial productions like foods, fermentation, textile, alcohol, paper, pharmaceutical and detergent. Screening of thermo-amylase bacteria in a hot spring needs to be done because the hot springs got potential as a source of thermostable amylase producing bacteria. Thermostable amylase that produced by natural bacteria (wild type) usually has activity that is not too high. The aim of this study is to get thermophile bacteria that produced thermostable amylase and increased the activity of thermostable amylase through optimization the condition of growth medium of thermophile bacteria. The result of this study is found 16 thermophile isolate bacteria and 3 thermophile bacteria that produced amylase. Thermostable amylase activity was high by using the agitation 125 rpm, wheat flour as the substrate at a concentration of 2% and amylase stability for 2 hours at 40-50°C. Thermostable amylase produced SMG9 of bacterial isolates that can be used in various industries.


Keywords: Isolation, Thermophile bacteria, Amylase, Optimization, Growth medium
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How to cite this article:

Gustina Indriati and Ruth Rize Paas Megahati. 2018. Isolation of Thermophilic Bacteria and Optimizing the Medium Growth Conditions.Int.J.Curr.Microbiol.App.Sci. 7(1): 1457-1464. doi: https://doi.org/10.20546/ijcmas.2018.701.177