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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 1275-1278
DOI: https://doi.org/10.20546/ijcmas.2018.701.155


Marker Validation in F2 Population of Rice (Oryza sativa L.) for Water and Nitrogen Use Efficiency
M.B. Boranayaka*, R. Lokesha, J.R. Diwan and Rajendragouda Patil
Department of Genetics and Plant Breeding, University of Agricultural Sciences, Raichur-584104, Karnataka, India
*Corresponding author
Abstract:

The new challenge of irrigated lowland rice is to sustain the needed increase in rice yields while reducing the requirements for water and N per unit of grain production. Validation of markers helps in determining the reliability and practical applicability of the markers in predicting the phenotype. In the present study, Simple sequence repeats (SSR) markers flanking WUE and NUE traits reported to explain high percentage phenotypic variation for their respective traits in RILs population from a cross Swarna x WAB450 were used for validation in F2 population individuals of BPT5204 x WAB450 and BPT5204 x Mysore Mallige crosses for the same traits for which they are already reported to be associated. Single marker analysis was done to with the help of student’s t test and by single factor ANOVA. Among the markers chosen for validating across the population, only few were found to be significantly associated with WUE and NUE traits in F2 mapping population individuals. This probably may be due to fact that these were detected in other mapping populations with different genetic background, and because the marker intervals may be quite large. The SSR markers RM518 and RM225 are associated with Water Use Efficiency and Nitrogen Use Efficiency in F2 mapping population of BPT5204 x WAB450 and BPT5204 x Mysore Mallige respectively. The per cent contribution of the significantly associated markers to total phenotypic variance were 48.26 and 38.74 percent with a p-value of 0.0135 and 0.0306 located on chromosome number 4 and 6 respectively. Low R2 values indicates large environmental component of variations. It can be further exploited in hybridization programme with MAS for improving WUE and NUE traits of rice genotypes.


Keywords: Oryza sativa, F2 population, Water and nitrogen use efficiency and marker validation
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How to cite this article:

Boranayaka, M.B., R. Lokesha, J.R. Diwan and Rajendragouda Patil. 2018. Marker Validation in F2 Population of Rice (Oryza sativa L.) for Water and Nitrogen Use Efficiency.Int.J.Curr.Microbiol.App.Sci. 7(1): 1275-1278. doi: https://doi.org/10.20546/ijcmas.2018.701.155