National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Present study aimed to screen commercial milk and milk products sold in the local markets of Bhubaneswar, Odisha for the presence of Mycobacterium avium subsp. paratuberculosis (MAP). A total of seventeen (17) samples comprising of commercial milk and milk products (curd, flavoured milk, lassi and milk powder) of leading commercial brands were purchased from the local markets in Bhubaneswar, Odisha. Samples were tested using antigen detection tests [milk microscopy (ZN staining), IS900 PCR, IS900 Real Time PCR, Indirect Fluorescent Antibody Test (iFAT)] and antibody detection test [Indigenous plate ELISA test (i_ELISA) by indigenous ELISA kit (provided by Animal Health Division of CIRG, Makhdoom)] in the Microbiology Laboratory, Animal Health Division, CIRG, Makhdoom. None (0%) of the 17 samples tested positive for MAP by any of above the tests, however, 12 were suspected for MAP by i_ELISA. The results indicated that the presence of MAP infection was very low, which sometimes becomes difficult to detect by multiple tests due to less sample size. Present pilot study underlined the need for screening of larger sample size especially raw and individual milk samples to arrive at conclusion as how serious is the threat of MAP in the livestock population of Odisha.
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