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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 753-761
DOI: https://doi.org/10.20546/ijcmas.2018.701.091


Bio-detection of Mycobacterium avium subspecies paratuberculosis in the Commercial Milk and Milk Products Sold in Bhubaneswar, Odisha, India
Sangram Biswal1, Shaswati Subhadarsini Pany2*, Shoor Vir Singh3, Niranjana Sahoo1, Manju Singh3, Sourabh Gupta3 and Kundan Kumar Chaubey3
1Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary Science and Animal Husbandry, O.U.A.T., Bhubaneswar, Odisha, India
2ICAR-International Centre for Foot and Mouth Disease, Arugul, Bhubaneswar-752050, Odisha, India
3Microbiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, PO- Farah, Mathura, India
*Corresponding author
Abstract:

Present study aimed to screen commercial milk and milk products sold in the local markets of Bhubaneswar, Odisha for the presence of Mycobacterium avium subsp. paratuberculosis (MAP). A total of seventeen (17) samples comprising of commercial milk and milk products (curd, flavoured milk, lassi and milk powder) of leading commercial brands were purchased from the local markets in Bhubaneswar, Odisha. Samples were tested using antigen detection tests [milk microscopy (ZN staining), IS900 PCR, IS900 Real Time PCR, Indirect Fluorescent Antibody Test (iFAT)] and antibody detection test [Indigenous plate ELISA test (i_ELISA) by indigenous ELISA kit (provided by Animal Health Division of CIRG, Makhdoom)] in the Microbiology Laboratory, Animal Health Division, CIRG, Makhdoom. None (0%) of the 17 samples tested positive for MAP by any of above the tests, however, 12 were suspected for MAP by i_ELISA. The results indicated that the presence of MAP infection was very low, which sometimes becomes difficult to detect by multiple tests due to less sample size. Present pilot study underlined the need for screening of larger sample size especially raw and individual milk samples to arrive at conclusion as how serious is the threat of MAP in the livestock population of Odisha.


Keywords: Johne’s disease, Milk, Milk products, Mycobacterium avium subspecies paratuberculosis, Odisha, Paratuberculosis, Prevalence
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How to cite this article:

Sangram Biswal, Shaswati Subhadarsini Pany, Shoor Vir Singh, Niranjana Sahoo, Manju Singh, Sourabh Gupta and Kundan Kumar Chaubey. 2018. Bio-detection of Mycobacterium avium subspecies paratuberculosis in the Commercial Milk and Milk Products Sold in Bhubaneswar, Odisha, IndiaInt.J.Curr.Microbiol.App.Sci. 7(1): 753-761. doi: https://doi.org/10.20546/ijcmas.2018.701.091