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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 745-752
DOI: https://doi.org/10.20546/ijcmas.2018.701.090


Prevalence of MRSA and Biofilm Associated Gene among Clinical Isolates of Staphylococci
Pradha Velu, Ramya Rengaraj*, Usha Sekar and Saleem
Department of Microbiology, MAPIMS, Kancheepuram, Tamil Nadu, India
*Corresponding author
Abstract:

Staphylococcus spp is a major cause of nosocomial and community-acquired infections and represents a significant burden on the healthcare system. Establishment of a mature biofilm, play an important role in the persistence of chronic infections and also decreases the susceptibility to antimicrobials and immune defenses, making these infections difficult to eradicate. MRSA and MR-CONS has become a major public health problem in both hospitals and communities. PVL has been reported to be an important marker for the highly pathogenic community acquired S. aureus infections. A rapid detection of these MRSA is very important for its treatment. Hence, this study was done to determine the prevalence of mec A, fem A and pvl gene using triplex PCR and its relation to phenotypic and genotypic presence of biofilm formation. We collected 73 clinical isolates of Staphylococcus spp from the tertiary care center. AST was performed and interpreted according to CLSI guidelines 2017. TCP method was used to detect the biofilm formation. Cefoxitin disk method was performed for detection of methicillin resistant followed by triplex PCR for the detection of mec A, fem A and pvl gene. A simplex PCR was done for detection biofilm encoding gene such as icaAD. Of the 73 isolates, 49 isolates were identified as S. aureus, 18 and 6 were S. epidermidis and S. saprophyticus respectively. The majority of the isolate was from pus and urine. By AST, highest resistance was observed for penicillin followed by erythromycin and co-trimoxazole. 51 isolates were considered as methicillin resistance by phenotypic method and by PCR. fem A was seen in 49 isolates and pvl was detected in 27 and 9 isolates of MRSA and MSSA respectively. 58 isolates were considered as strong biofilm producers and 15 were non-biofilm producers by TCP method. PCR detected icaAD in 31 isolates.


Keywords: Staphylococcus, MRSA, Bioflim, mecA gene, CoNS
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How to cite this article:

Pradha Velu, Ramya Rengaraj, Usha Sekar and Saleem. 2018. Prevalence of MRSA and Biofilm Associated Gene among Clinical Isolates of Staphylococci.Int.J.Curr.Microbiol.App.Sci. 7(1): 745-752. doi: https://doi.org/10.20546/ijcmas.2018.701.090