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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 687-699
DOI: https://doi.org/10.20546/ijcmas.2018.701.084


Quantification, Antibacterial Assay and Cytotoxic Effect of Combretastatin, an Anticancer Compound from Three Indian Combretum species
Arundhati Das, K.C. Samal, A.B. Das and G.R. Rout*
Department of Agricultural Biotechnology, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar -751003, Odisha, India
*Corresponding author
Abstract:

Combretastatin A4 is a natural stilbenoid phenol derived from South African Bushwillow (Combretum caffrum). Combretum albidum, Crombretum densiflorum and Combretum roxburghii (Family: Combretaceae) are the unexplored medicinal plant in the Indian medicinal system. According to ethno-botanical information, the leaves, barks and fruits Combretum species have antimicrobial and anti-tumor activities and extensively used in the treatment of syphilis, abdominal pains, conjunctivitis, diarrhoea, toothache, peptic ulcer, dysentery, Jaundice, skin, heart and cancer diseases. The present study was undertaken to ascertain the presence of anticancer phyto-constituent in the Indian Combretum species and its further utilization in the treatment of tumor. The phyto-chemical constituents of the leaf and bark extracts were recognized with reference to standard on the same HPTLC plate. The leaf extracts exhibited higher quantity of combretastatins than the bark samples. The combretastatin content was recorded the highest (182 µg/g) in the leaf extract of C. albidum. Antibacterial activity was studied by disk diffusion assay against seven bacterial strains. The leaf extract of C. densiflorum resulted higher level of zone of inhibition against Bacillus subtilis (19 mm), Escherichia coli (18 mm), Salmonella enteric (18 mm), Streptococcus pneumoniae (16 mm), Staphylococcus aurius (13 mm), Pseudomonas aeruginosa (12 mm) and Mycococcus luteus (12 mm). Lower antimicrobial activities were recorded by the leaf and bark extracts of C. albidum. The carcinogenic effect of methanolic crude extracts on Allium cepa cells inducing chromosomal aberrations like chromosomal clumping, sticky bridge, laggard, early separation, late separation, chromosome break (fragmentation) and erosion (heavy fragmentation) were recorded. The methanolic leaf extract of Combretum albidum exhibited the highest percentage of chromosomal aberration such as sticky bridge in metaphase which demonstrated the strongest cytotoxic effect in the root meristem cells. These results suggest that the methanolic extract of Combretum species has antimicrobial and cytotoxic activities that support the ethano-pharmacological and anti-tumor uses of these plants.


Keywords: Combretastatin, HPTLC, Cytotoxic effects, Antibacterial activity, Anti-tumor, Cancer
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How to cite this article:

Arundhati Das, K.C. Samal, A.B. Das and Rout, G.R. 2018. Quantification, Antibacterial Assay and Cytotoxic Effect of Combretastatin, an Anticancer Compound from Three Indian Combretum species.Int.J.Curr.Microbiol.App.Sci. 7(1): 687-699. doi: https://doi.org/10.20546/ijcmas.2018.701.084