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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 670-680
DOI: https://doi.org/10.20546/ijcmas.2018.701.082


Molecular Identification, Production and Optimization of Pectinase by using Stenotrophomonas maltophilia P9 Isolated from Algal Biomass of Himachal Pradesh, India
Poonam Sharma1* and Nivedita Sharma2
1Microbiology research laboratory, Department of Basic sciences, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni (Solan) Himachal Pradesh 173230, India
2Department of Basic sciences, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni (Solan) Himachal Pradesh 173230, India
*Corresponding author
Abstract:

The demand for enzymes in the global market is projected to rise at a fast pace in recent years. There has been a great increase in industrial applications of pectinase owing to their significant biotechnological uses. Therefore the study was undertaken with aims of; screening pectinase producing bacteria from Rhizoclonium sp. algal biomass collected from different parts of Himachal Pradesh. The isolate P9 with high potential pectinase activity was identified molecularly by sequencing 16s rDNA region of the isolate and identified as Stenotrophomonas maltophilia P9 was selected for production. Various optimization steps by using classical one factor at a time approach (OFAT) were carried out to make the production of pectinase enzyme cost effective and commercially viable. The various parameters studied for pectinase production were different media, medium pH, temperature, inoculum size and incubation period. The maximum pectinase production was observed using Adessina et al., (2013) media at initial pH of 5.0, at 30oC with 10% inoculum size followed by incubation period of 72 h. In the present study, an attempt was made to isolate efficient pectinase producing bacteria from least explored source of enzymes i.e. algal biomass of Himachal Pradesh and can be further used for various biotechnological applications.


Keywords: Pectinase, Optimization, Algal biomass, Molecular identification
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How to cite this article:

Poonam Sharma and Nivedita Sharma. 2018. Molecular Identification, Production and Optimization of Pectinase by using Stenotrophomonas maltophilia P9 Isolated from Algal Biomass of Himachal Pradesh, India.Int.J.Curr.Microbiol.App.Sci. 7(1): 670-680. doi: https://doi.org/10.20546/ijcmas.2018.701.082